Section: Zoology
Topic: Evolution, Genetics/genomics

Museomics of Carabus giant ground beetles shows an Oligocene origin and in situ alpine diversification

Pauli, Marie T.1, 2  ; Gauthier, Jérémy1, 2  ; Labédan, Marjorie1, 3 ; Blanc, Mickael1 ; Bilat, Julia1 ; Toussaint, Emmanuel F. A.1  
1 Natural History Museum of Geneva, Geneva, Switzerland
2 Cantonal Museum of Natural Sciences, Lausanne, Switzerland
3 Department of Ecology and Evolution, University of Lausanne, Lausanne, Switzerland

10.24072/pcjournal.445 - Peer Community Journal, Volume 4 (2024), article no. e70.

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The development of museomics represents a major paradigm shift in the use of natural history collection specimens for systematics and evolutionary biology. New approaches in this field allow the sequencing of hundreds to thousands of loci from across the genome using historical DNA. HyRAD-X, a recently introduced capture method using bench-top designed probes, has proved very efficient for recovering genomic-scale datasets using natural history collection specimens. Using this technique at both the intra- and interspecific levels, we infer the most robust phylogeny to date for Arcifera, an ecologically and morphologically diverse clade of Carabus giant ground beetles. We successfully generated a genomic dataset of up to 1965 HyRAD-X loci for all described species, permitting inference of a robust dated phylogenomic tree for this clade. Our species delimitation and population genomic analyses suggest that the current classification for Arcifera is in line with its evolutionary history. Our results suggest an origin of Arcifera in the late Oligocene followed by speciation events during the warm mid-Miocene unlinked to Pleistocene glaciations. The dynamic paleogeographic history of the Palearctic region likely contributed to the diversification of this lineage with a relatively ancient colonization of the proto-Alps followed by in situ speciation where most species of Arcifera are currently found sometimes syntopically likely as a result of post-glaciations secondary contacts.

Published online:
DOI: 10.24072/pcjournal.445
Type: Research article
Keywords: Arcifera, Beetle evolution, Carabinae, Historical DNA, HyRAD-X, Phylogenomics, Palearctic biogeography, Pleistocene glaciations, Arcifera, Beetle evolution, Carabinae, Historical DNA, HyRAD-X, Phylogenomics, Palearctic biogeography, Pleistocene glaciations

Pauli, Marie T. 1, 2; Gauthier, Jérémy 1, 2; Labédan, Marjorie 1, 3; Blanc, Mickael 1; Bilat, Julia 1; Toussaint, Emmanuel F. A. 1

1 Natural History Museum of Geneva, Geneva, Switzerland
2 Cantonal Museum of Natural Sciences, Lausanne, Switzerland
3 Department of Ecology and Evolution, University of Lausanne, Lausanne, Switzerland
License: CC-BY 4.0
Copyrights: The authors retain unrestricted copyrights and publishing rights 
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     title = {Museomics of {\protect\emph{Carabus}} giant ground beetles shows an {Oligocene} origin and \protect\emph{in situ }alpine diversification},
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Pauli, Marie T.; Gauthier, Jérémy; Labédan, Marjorie; Blanc, Mickael; Bilat, Julia; Toussaint, Emmanuel F. A. Museomics of Carabus giant ground beetles shows an Oligocene origin and in situ alpine diversification. Peer Community Journal, Volume 4 (2024), article  no. e70. doi : 10.24072/pcjournal.445. https://peercommunityjournal.org/articles/10.24072/pcjournal.445/

PCI peer reviews and recommendation, and links to data, scripts, code and supplementary information: 10.24072/pci.zool.100319

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The recommender in charge of the evaluation of the article and the reviewers declared that they have no conflict of interest (as defined in the code of conduct of PCI) with the authors or with the content of the article.

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