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Section: Genomics
Topic:
Genetics/Genomics,
Microbiology,
Ecology
Gut microbial ecology of Xenopus tadpoles across life stages
10.24072/pcjournal.53 - Peer Community Journal, Volume 1 (2021), article no. e41.
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Background: The microorganism world living in amphibians is still largely under-represented and under-studied in the literature. Among anuran amphibians, African clawed frogs of the Xenopus genus stand as well-characterized models with an in-depth knowledge of their developmental biological processes including their metamorphosis. In this study, we analyzed the succession of microbial communities and their activities across diverse body habitats of Xenopus tropicalis using different approaches including flow cytometry and 16s rDNA gene metabarcoding. We also evaluated the metabolic capacity of the premetamorphic tadpoles gut microbiome using metagenomic and metatranscriptomic sequencing. Results: We analyzed the bacterial components of the Xenopus gut microbiota, the adult gut biogeography, the succession of communities during ontogeny, the impact of the alimentation in shaping the tadpoles gut bacterial communities and the transmission of skin and fecal bacteria to the eggs. We also identified the most active gut bacteria and their metabolic contribution to tadpole physiology including carbohydrate breakdown, nitrogen recycling, essential amino-acids and vitamin biosynthesis. Conclusions: We present a comprehensive new microbiome dataset of a laboratory amphibian model. Our data provide evidences that studies on the Xenopus tadpole model can shed light on the interactions between a vertebrate host and its microbiome. We interpret our findings in light of bile acids being key molecular components regulating the gut microbiome composition during amphibian development and metamorphosis. Further studies into the metabolic interactions between amphibian tadpoles and their microbiota during early development and metamorphosis should provide useful information on the evolution of host-microbiota interactions in vertebrates.
Type: Research article
Scalvenzi, Thibault 1; Clavereau, Isabelle 1; Bourge, Mickaël 2; Pollet, Nicolas 1
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@article{10_24072_pcjournal_53, author = {Scalvenzi, Thibault and Clavereau, Isabelle and Bourge, Micka\"el and Pollet, Nicolas}, title = {Gut microbial ecology of {\protect\emph{Xenopus}} tadpoles across life stages}, journal = {Peer Community Journal}, eid = {e41}, publisher = {Peer Community In}, volume = {1}, year = {2021}, doi = {10.24072/pcjournal.53}, url = {https://peercommunityjournal.org/articles/10.24072/pcjournal.53/} }
TY - JOUR AU - Scalvenzi, Thibault AU - Clavereau, Isabelle AU - Bourge, Mickaël AU - Pollet, Nicolas TI - Gut microbial ecology of Xenopus tadpoles across life stages JO - Peer Community Journal PY - 2021 VL - 1 PB - Peer Community In UR - https://peercommunityjournal.org/articles/10.24072/pcjournal.53/ DO - 10.24072/pcjournal.53 ID - 10_24072_pcjournal_53 ER -
%0 Journal Article %A Scalvenzi, Thibault %A Clavereau, Isabelle %A Bourge, Mickaël %A Pollet, Nicolas %T Gut microbial ecology of Xenopus tadpoles across life stages %J Peer Community Journal %D 2021 %V 1 %I Peer Community In %U https://peercommunityjournal.org/articles/10.24072/pcjournal.53/ %R 10.24072/pcjournal.53 %F 10_24072_pcjournal_53
Scalvenzi, Thibault; Clavereau, Isabelle; Bourge, Mickaël; Pollet, Nicolas. Gut microbial ecology of Xenopus tadpoles across life stages. Peer Community Journal, Volume 1 (2021), article no. e41. doi : 10.24072/pcjournal.53. https://peercommunityjournal.org/articles/10.24072/pcjournal.53/
PCI peer reviews and recommendation, and links to data, scripts, code and supplementary information: 10.24072/pci.genomics.100005
Conflict of interest of the recommender and peer reviewers:
The recommender in charge of the evaluation of the article and the reviewers declared that they have no conflict of interest (as defined in the code of conduct of PCI) with the authors or with the content of the article.
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