Section: Animal Science
Topic: Agricultural sciences, Cell biology

The impact of housing conditions on porcine mesenchymal stromal/stem cell populations differ between adipose tissue and skeletal muscle

10.24072/pcjournal.109 - Peer Community Journal, Volume 2 (2022), article no. e25.

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Background. In pigs, the ratio between lean mass and fat mass in the carcass determines production efficiency and is strongly influenced by the number and size of cells in tissues. During growth, the increase in the number of cells results from the recruitment of different populations of multipotent mesenchymal stromal/stem cells (MSCs) residing in the tissues. We hypothesized that the impact of hygiene of housing conditions during growth on the proportions of MSCs in adipose tissue and skeletal muscle may differ between pigs with different residual feed intake (RFI), a measure of feed efficiency. Methods. At the age of 12 weeks, Large White pigs from two lines divergently selected for low and high RFI were housed in two contrasting hygiene conditions (good vs poor). After six weeks, pigs were slaughtered (n = 30; 5-9/group). Samples of subcutaneous adipose tissue and longissimus skeletal muscle were collected, and cells from the stromal vascular fraction (SVF), which includes mesenchymal stromal/stem cell populations, were isolated from each tissue. Adipose and muscle cell populations from the SVF were phenotyped by flow cytometry using antibodies that targeted different cell surface markers (CD45 to separate hematopoietic cells from MSCs; CD34, CD38, CD56 and CD140a to identify MSC populations with adipogenic and/or myogenic potential). Results. Adipose tissue and muscle shared some common MSC populations although MSC diversity was higher in muscle than in adipose tissue. In muscle, the CD45-CD56+CD34-CD140a+ and CD45-CD56+CD34+CD140a+ cell populations were abundant. Of these two cell populations, only the proportions of CD45-CD56+CD34+CD140a+ cells increased (P ≤ 0.05) in pigs housed in poor hygiene as compared with pigs in good hygiene conditions. For the CD45-CD56-CD34- cell population, present in low proportion, there was an interaction between hygiene condition and genetic line (P < 0.05) with a decrease in low RFI pigs housed in poor hygiene conditions. In adipose tissue, the two abundant MSC populations were CD45-CD56-CD34- and CD45-CD56+CD34-. The proportion of CD45-CD56-CD34- cells increased (P < 0.05) whereas the proportion of CD45-CD56+CD34- tended to decrease (P < 0.1) in pigs housed in poor conditions. This study shows that the proportions of some MSC populations were affected by hygiene of housing conditions in a tissue-dependent manner in pigs of both RFI lines. It suggests that MSCs may play a significant role in adipose tissue and skeletal muscle homeostasis and may influence later growth and body composition in growing animals.

Published online:
DOI: 10.24072/pcjournal.109
Type: Research article

Quéméner, Audrey 1; Dessauge, Frédéric 1; Perruchot, Marie-Hélène 1; Le Floc’h, Nathalie 1; Louveau, Isabelle 1

1 PEGASE, INRAE, Institut Agro, Saint-Gilles, France
License: CC-BY 4.0
Copyrights: The authors retain unrestricted copyrights and publishing rights
     author = {Qu\'em\'ener, Audrey and Dessauge, Fr\'ed\'eric and Perruchot, Marie-H\'el\`ene and Le Floc{\textquoteright}h, Nathalie and Louveau, Isabelle},
     title = {The impact of housing conditions on porcine mesenchymal stromal/stem cell populations differ between adipose tissue and skeletal muscle},
     journal = {Peer Community Journal},
     eid = {e25},
     publisher = {Peer Community In},
     volume = {2},
     year = {2022},
     doi = {10.24072/pcjournal.109},
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AU  - Quéméner, Audrey
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AU  - Le Floc’h, Nathalie
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JO  - Peer Community Journal
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%0 Journal Article
%A Quéméner, Audrey
%A Dessauge, Frédéric
%A Perruchot, Marie-Hélène
%A Le Floc’h, Nathalie
%A Louveau, Isabelle
%T The impact of housing conditions on porcine mesenchymal stromal/stem cell populations differ between adipose tissue and skeletal muscle
%J Peer Community Journal
%D 2022
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%R 10.24072/pcjournal.109
%F 10_24072_pcjournal_109
Quéméner, Audrey; Dessauge, Frédéric; Perruchot, Marie-Hélène; Le Floc’h, Nathalie; Louveau, Isabelle. The impact of housing conditions on porcine mesenchymal stromal/stem cell populations differ between adipose tissue and skeletal muscle. Peer Community Journal, Volume 2 (2022), article  no. e25. doi : 10.24072/pcjournal.109.

PCI peer reviews and recommendation, and links to data, scripts, code and supplementary information: 10.24072/pci.animsci.100011

Conflict of interest of the recommender and peer reviewers:
The recommender in charge of the evaluation of the article and the reviewers declared that they have no conflict of interest (as defined in the code of conduct of PCI) with the authors or with the content of the article.

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