Infections

Development of nine microsatellite loci for Trypanosoma lewisi, a potential human pathogen in Western Africa and South-East Asia, and preliminary population genetics analyses

10.24072/pcjournal.188 - Peer Community Journal, Volume 2 (2022), article no. e69.

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Trypanosoma lewisi belongs to the so-called atypical trypanosomes that occasionally affect humans. It shares the same hosts and flea vector of other medically relevant pathogenic agents as Yersinia pestis, the agent of plague. Increasing knowledge on the population structure (reproductive mode, population size, dispersal) of this parasite thus represents a challenging but important issue. The use of polymorphic genetic markers, together with suitable population genetics tools, is a convenient way to achieve such objectives. To date, the population biology of T. lewisi is poorly known and, to our knowledge, no population genetics studies have ever been conducted. Here, we present the development of nine microsatellite markers of this species. We investigated their polymorphism in different countries from Africa and South-East Asia from DNAs extracted from the spleen of their rodent reservoirs (essentially rat species). Several amplification problems arose, especially with South-East Asian individuals. This led to retain only those individuals with complete genotypes (most of them originating from West Africa, notably Cotonou, Benin) to ensure an optimal estimate of heterozygosity. Our results pointed towards a mainly (at least 95-99%) clonal mode of propagation, a strong subdivision at the smallest scale available (i.e., urban neighborhoods, i.e. 0.250 km²), and a generation time most probably shorter than 4 months. In future studies, more extensive sampling at smaller geographic scales (i.e., households), within a one- or two-months window and with improved amplification conditions, should lead to a more precise picture of the fine population structure of this parasite.

Published online:
DOI: 10.24072/pcjournal.188
Ségard, Adeline 1; Romero, Audrey 1; Ravel, Sophie 1; Truc, Philippe 1; Dobigny, Gauthier 2; Gauthier, Philippe 2; Etougbetche, Jonas 3; Dossou, Henri-Joel 3; Badou, Sylvestre 3; Houéménou, Gualbert 3; Morand, Serge 4, 5; Chaisiri, Kittipong 6; Noûs, Camille 7; de Meeûs, Thierry 1

1 Univ Montpellier, Cirad, IRD, Intertryp, Montpellier, France
2 CBGP, IRD, Montpellier, France
3 URIB, LARBA, EPAC, Univ. Abomey-Calavi, Cotonou, Benin
4 MIVEGEC, CNRS, IRD, Montpellier Université, Montpellier, France
5 Faculty of Veterinary, Kasetsart University, Bangkok, Thailand
6 Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
7 Cogitamus laboratory, France
License: CC-BY 4.0
Copyrights: The authors retain unrestricted copyrights and publishing rights
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     author = {S\'egard, Adeline and Romero, Audrey and Ravel, Sophie and Truc, Philippe and Dobigny, Gauthier and Gauthier, Philippe and Etougbetche, Jonas and Dossou, Henri-Joel and Badou, Sylvestre and Hou\'em\'enou, Gualbert and Morand, Serge and Chaisiri, Kittipong and No\^us, Camille and de Mee\^us, Thierry},
     title = {Development of nine microsatellite loci for {<i>Trypanosoma} lewisi</i>, a potential human pathogen in {Western} {Africa} and {South-East} {Asia,} and preliminary population genetics analyses},
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%A Etougbetche, Jonas
%A Dossou, Henri-Joel
%A Badou, Sylvestre
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%A Noûs, Camille
%A de Meeûs, Thierry
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Ségard, Adeline; Romero, Audrey; Ravel, Sophie; Truc, Philippe; Dobigny, Gauthier; Gauthier, Philippe; Etougbetche, Jonas; Dossou, Henri-Joel; Badou, Sylvestre; Houéménou, Gualbert; Morand, Serge; Chaisiri, Kittipong; Noûs, Camille; de Meeûs, Thierry. Development of nine microsatellite loci for Trypanosoma lewisi, a potential human pathogen in Western Africa and South-East Asia, and preliminary population genetics analyses. Peer Community Journal, Volume 2 (2022), article  no. e69. doi : 10.24072/pcjournal.188. https://peercommunityjournal.org/articles/10.24072/pcjournal.188/

Peer reviewed and recommended by PCI : 10.24072/pci.infections.100004

Conflict of interest of the recommender and peer reviewers:
The recommender in charge of the evaluation of the article and the reviewers declared that they have no conflict of interest (as defined in the code of conduct of PCI) with the authors or with the content of the article.

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