Section: Ecotoxicology & Environmental Chemistry
Topic: Environmental sciences

Soot and charcoal as reservoirs of extracellular DNA

10.24072/pcjournal.207 - Peer Community Journal, Volume 2 (2022), article no. e80.

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The vast potential of using sediment adsorbed DNA as a window to past and present biodiversity rely on the ability of solid surfaces to adsorb environmental DNA. However, a comprehensive insight into DNA adsorption at surfaces in general is lacking. Soot and charcoal are carbonaceous materials widespread in the environment where they readily can come in contact with extracellular DNA shed from organisms. Using batch adsorption, we measured DNA adsorption capacity at soot and charcoal as a function of solution composition, time and DNA length. We observed that the adsorption capacity for DNA is highest at low pH, that it increases with solution concentration and cation valency and that the activation energy for DNA adsorption at both soot and charcoal is ~50 kJmol-1, suggesting strong binding. We demonstrate how the interaction between DNA and soot and charcoal partly occurs via terminal base pairs, suggesting that, besides electrostatic forces, hydrophobic interactions play an important role in binding. The large adsorption capacities and strong binding of DNA to soot and charcoal are features important for eDNA research and provide a motivation for use of carbonaceous materials from, e.g., anthropogenic pollution or wildfire as sources of biodiversity information.

Published online:
DOI: 10.24072/pcjournal.207
Type: Research article
Jelavić, Stanislav 1, 2; Thygesen, Lisbeth G. 3; Magnin, Valérie 2; Findling, Nathaniel 2; Müller, Sascha 4; Meklesh, Viktoriia 5; Sand, Karina K. 1

1 Centre for Geogenetics, GLOBE Institute, University of Copenhagen, Øster Voldgade 5–7, 1350 Copenhagen, Denmark
2 Université Grenoble Alpes, Université Savoie Mont Blanc, CNRS, IRD, Université Gustave Eiffel, ISTerre, F-38000 Grenoble, France
3 University of Copenhagen, Department of Geoscience and Natural Resource Management, Rolighedsvej 23, 1958 Frederiksberg C, Denmark
4 University of Copenhagen, Department of Geosciences and Natural Resource Management, Øster Voldgade 10, 1350 Copenhagen K, Copenhagen, Denmark
5 Centre for Environmental and Climate Science, Lund University, Sölvegatan 37, 223 62 Lund, Sweden
License: CC-BY 4.0
Copyrights: The authors retain unrestricted copyrights and publishing rights
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     author = {Jelavi\'c, Stanislav and Thygesen, Lisbeth G. and Magnin, Val\'erie and Findling, Nathaniel and M\"uller, Sascha and Meklesh, Viktoriia and Sand, Karina K.},
     title = {Soot and charcoal as reservoirs of extracellular {DNA}},
     journal = {Peer Community Journal},
     eid = {e80},
     publisher = {Peer Community In},
     volume = {2},
     year = {2022},
     doi = {10.24072/pcjournal.207},
     url = {https://peercommunityjournal.org/articles/10.24072/pcjournal.207/}
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%A Thygesen, Lisbeth G.
%A Magnin, Valérie
%A Findling, Nathaniel
%A Müller, Sascha
%A Meklesh, Viktoriia
%A Sand, Karina K.
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Jelavić, Stanislav; Thygesen, Lisbeth G.; Magnin, Valérie; Findling, Nathaniel; Müller, Sascha; Meklesh, Viktoriia; Sand, Karina K. Soot and charcoal as reservoirs of extracellular DNA. Peer Community Journal, Volume 2 (2022), article  no. e80. doi : 10.24072/pcjournal.207. https://peercommunityjournal.org/articles/10.24072/pcjournal.207/

PCI peer reviews and recommendation, and links to data, scripts, code and supplementary information: 10.24072/pci.ecotoxenvchem.100003

Conflict of interest of the recommender and peer reviewers:
The recommender in charge of the evaluation of the article and the reviewers declared that they have no conflict of interest (as defined in the code of conduct of PCI) with the authors or with the content of the article.

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