Comparative abundance and diversity of populations of the Pseudomonas syringae and Soft Rot Pectobacteriaceae species complexes throughout the Durance River catchment from its French Alps sources to its delta

10.24072/pcjournal.317 - Peer Community Journal, Volume 3 (2023), article no. e88.

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Rivers, creeks, streams are integrators of biological, chemical and physical processes occurring in a catchment linking land cover from the headwaters to the outlet.  The dynamics of human and animal pathogens in catchments have been widely studied in a large variety of contexts allowing the optimization of disease risk reduction. In parallel, there is an emerging awareness that crop pathogens might also be disseminated via surface waters especially when they are used for irrigation. However, there are no studies on the extent to which potential plant pathogens are present – nor about their dynamics - along the full course of a catchment. Here we have compared the seasonal dynamics of populations of the Pseudomonas syringae (Psy) and the Soft Rot Pectobacteriaceae (SRP) species complexes along a 270 km stretch of the Durance River from the upstream alpine reaches to the downstream agricultural production areas at the confluence with the Rhone River at Avignon. Among 168 samples collected at 21 sites in fall, winter, spring and summer of 2016 and 2017, Psy strains were detected at all sampling sites and in 156 of the samples at population densities up to 105 bacteria L-1. In contrast, SRP strains were detected in 98 of the samples, mostly from the southern part of the river, at population densities that did not exceed 3 ´ 104 bacteria L-1. Among the biological and chemical parameters that were characterized at each sampling site, temperature was the only factor that explained a significant amount of the variability in population size for both species complexes. Psy densities decreased with increasing temperature whereas SRP densities increased with increasing temperature. River-borne populations of SRP were composed mainly of Pectobacterium versatile and P. aquaticum that have little known epidemiological importance. Only a few strains of Pectobacterium and Dickeya species reputed for their epidemiological impact were observed. In contrast, Psy populations at all sites were dominated by a genetic lineage of phylogroup 2 known from other studies for its broad host range and its geographic and habitat ubiquity. Our observations suggest that surveillance of river water for SRP could be leveraged to signal diagnostic and management reactions to avoid disease outbreaks.  In contrast, the constant presence of Psy throughout the catchment in absence of regular and widespread disease outbreaks due to this group of bacteria suggests that surveillance should focus on future changes in land use, river water conditions and agronomic practices that could destabilize the mechanisms currently holding Psy outbreaks in check.

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DOI: 10.24072/pcjournal.317
Keywords: plant pathogenic bacteria, microbial ecology, hydrology, surface waters, irrigation
Morris, C.E. 1; Lacroix, C. 1; Chandeysson, C. 1; Guilbaud, C. 1; Monteil, C. 1; Piry, S. 1; Rochelle Newall, E. 2; Fiorini, S. 3; Van Gijsegem, F. 2; Barny, M.A. 2; Berge, O. 1

1 INRAE, Pathologie Végétale, F-84140, Montfavet, France
2 Sorbonne Université, INRAE, IRD, CNRS, UPEC, Institute of Ecology and Environmental Sciences-Paris (iEES-Paris), 4 place Jussieu, F-75252 Paris, France
3 Ecole Normale Supérieure, PSL Research University, CNRS, Centre de recherche en écologie expérimentale et prédictive (CEREEP-Ecotron Ile De France), UAR 3194, 11 chemin de Busseau, F-77140 Saint-Pierre-lès-Nemours, France
License: CC-BY 4.0
Copyrights: The authors retain unrestricted copyrights and publishing rights
     author = {Morris, C.E. and Lacroix, C. and Chandeysson, C. and Guilbaud, C. and Monteil, C. and Piry, S. and Rochelle Newall, E. and Fiorini, S. and Van Gijsegem, F. and Barny, M.A. and Berge, O.},
     title = {Comparative abundance and diversity of populations of the {\protect\emph{Pseudomonas} syringae} and {Soft} {Rot} {\protect\emph{Pectobacteriaceae}} species complexes throughout the {Durance} {River} catchment from its {French} {Alps} sources to its delta},
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Morris, C.E.; Lacroix, C.; Chandeysson, C.; Guilbaud, C.; Monteil, C.; Piry, S.; Rochelle Newall, E.; Fiorini, S.; Van Gijsegem, F.; Barny, M.A.; Berge, O. Comparative abundance and diversity of populations of the Pseudomonas syringae and Soft Rot Pectobacteriaceae species complexes throughout the Durance River catchment from its French Alps sources to its delta. Peer Community Journal, Volume 3 (2023), article  no. e88. doi : 10.24072/pcjournal.317. https://peercommunityjournal.org/articles/10.24072/pcjournal.317/

Peer reviewed and recommended by PCI : 10.24072/pci.microbiol.100006

Conflict of interest of the recommender and peer reviewers:
The recommender in charge of the evaluation of the article and the reviewers declared that they have no conflict of interest (as defined in the code of conduct of PCI) with the authors or with the content of the article.

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