Chikungunya intra-vector dynamics in Aedes albopictus from Lyon (France) upon exposure to a human viremia-like dose range reveals vector barrier’s permissiveness and supports local epidemic potential

10.24072/pcjournal.326 - Peer Community Journal, Volume 3 (2023), article no. e96.

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Arbovirus emergence and epidemic potential, as approximated by the vectorial capacity formula, depends on host and vector parameters, including the vector’s intrinsic ability to replicate then transmit the pathogen known as vector competence. Vector competence is a complex, time-dependent, quantitative phenotype influenced by biotic and abiotic factors. A combination of experimental and modelling approaches is required to assess arbovirus intra-vector dynamics and estimate epidemic potential. In this study, we measured infection, dissemination, and transmission dynamics of chikungunya virus (CHIKV) in a field-derived Aedes albopictus population (Lyon, France) after oral exposure to a range of virus doses spanning human viraemia. Statistical modelling indicates rapid and efficient CHIKV progression in the vector mainly due to an absence of a dissemination barrier, with 100% of the infected mosquitoes ultimately exhibiting a disseminated infection, regardless of the virus dose. Transmission rate data revealed a time-dependent, but overall weak, transmission barrier, with individuals transmitting as soon as 2 days post-exposure (dpe) and >50% infectious mosquitoes at 6 dpe for the highest dose. Based on these experimental intra-vector dynamics data, epidemiological simulations conducted with an agent-based model showed that even at low mosquito biting rates, CHIKV could trigger outbreaks locally. Together, this reveals the epidemic potential of CHIKV upon transmission by Aedes albopictus in mainland France.

Published online:
DOI: 10.24072/pcjournal.326
Keywords: Arbovirus; vector; mosquito; Aedes albopictus; chikungunya virus; epidemiology; vector competence; modelisation
Viginier, Barbara 1; Cappuccio, Lucie 1; Garnier, Céline 1; Martin, Edwige 2; Maisse, Carine 1; Valiente Moro, Claire 2; Minard, Guillaume 2; Fontaine, Albin 3, 4, 5; Lequime, Sébastian 6; Ratinier, Maxime 1; Arnaud, Frédérick 1; Raquin, Vincent 1

1 IVPC UMR754, INRAE, Universite Claude Bernard Lyon 1, EPHE, PSL Research University, F-69007 Lyon, France
2 Universite Claude Bernard Lyon 1, CNRS, INRAE, VetAgro Sup, UMR Ecologie Microbienne, F-69622 Villeurbanne, France
3 Unité Parasitologie et Entomologie, Département Microbiologie et maladies infectieuses, Institut de Recherche Biomédicale des Armées (IRBA), Marseille, France
4 Aix Marseille Univ, IRD, SSA, AP-HM, UMR Vecteurs–Infections Tropicales et Méditerranéennes (VITROME), Marseille, France
5 IHU Méditerranée Infection, Marseille, France
6 Cluster of Microbial Ecology, Groningen Institute for Evolutionary Life Sciences, University of Groningen, Groningen, The Netherlands
License: CC-BY 4.0
Copyrights: The authors retain unrestricted copyrights and publishing rights
     author = {Viginier, Barbara and Cappuccio, Lucie and Garnier, C\'eline and Martin, Edwige and Maisse, Carine and  Valiente Moro, Claire and Minard, Guillaume and Fontaine, Albin and Lequime, S\'ebastian and Ratinier, Maxime and Arnaud, Fr\'ed\'erick and Raquin, Vincent},
     title = {Chikungunya intra-vector dynamics {in~\protect\emph{Aedes} albopictus}~from {Lyon} {(France)} upon exposure to a human viremia-like dose range reveals vector barrier{\textquoteright}s permissiveness and supports local epidemic potential},
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     language = {en},
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AU  - Cappuccio, Lucie
AU  - Garnier, Céline
AU  - Martin, Edwige
AU  - Maisse, Carine
AU  -  Valiente Moro, Claire
AU  - Minard, Guillaume
AU  - Fontaine, Albin
AU  - Lequime, Sébastian
AU  - Ratinier, Maxime
AU  - Arnaud, Frédérick
AU  - Raquin, Vincent
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%A Cappuccio, Lucie
%A Garnier, Céline
%A Martin, Edwige
%A Maisse, Carine
%A  Valiente Moro, Claire
%A Minard, Guillaume
%A Fontaine, Albin
%A Lequime, Sébastian
%A Ratinier, Maxime
%A Arnaud, Frédérick
%A Raquin, Vincent
%T Chikungunya intra-vector dynamics in Aedes albopictus from Lyon (France) upon exposure to a human viremia-like dose range reveals vector barrier’s permissiveness and supports local epidemic potential
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Viginier, Barbara; Cappuccio, Lucie; Garnier, Céline; Martin, Edwige; Maisse, Carine;  Valiente Moro, Claire; Minard, Guillaume; Fontaine, Albin; Lequime, Sébastian; Ratinier, Maxime; Arnaud, Frédérick; Raquin, Vincent. Chikungunya intra-vector dynamics in Aedes albopictus from Lyon (France) upon exposure to a human viremia-like dose range reveals vector barrier’s permissiveness and supports local epidemic potential. Peer Community Journal, Volume 3 (2023), article  no. e96. doi : 10.24072/pcjournal.326.

Peer reviewed and recommended by PCI : 10.24072/pci.infections.100091

Conflict of interest of the recommender and peer reviewers:
The recommender in charge of the evaluation of the article and the reviewers declared that they have no conflict of interest (as defined in the code of conduct of PCI) with the authors or with the content of the article.

[1] Aliota, M.; Walker, E.; Yepes, A.; Velez, I.; Christensen, B.; Osorio, J. The wMel Strain of Wolbachia Reduces Transmission of Chikungunya Virus in Aedes aegypti, PLoS neglected tropical diseases, Volume 10, p. 0004677 | DOI

[2] Appassakij, H.; Khuntikij, P.; Kemapunmanus, M.; Wutthanarungsan, R.; Silpapojakul, K. Viremic profiles in CHIKV‐infected cases, Transfusion, Volume 53, pp. 2567-2574 | DOI

[3] Armstrong, P.; Ehrlich, H.; Magalhaes, T.; Miller, M.; Conway, P.; Bransfield, A.; Misencik, M.; Gloria-Soria, A.; Warren, J.; Andreadis, T.; Shepard, J.; Foy, B.; Pitzer, V.; Brackney, D. Successive blood meals enhance virus dissemination within mosquitoes and increase transmission potential, Nature microbiology, pp. 1-9 | DOI

[4] Aubry, F.; Dabo, S.; Manet, C.; Filipović, I.; Rose, N.; Miot, E.; Martynow, D.; Baidaliuk, A.; Merkling, S.; Dickson, L.; Crist, A.; Anyango, V.; Romero-Vivas, C.; Vega-Rúa, A.; Dusfour, I.; Jiolle, D.; Paupy, C.; Mayanja, M.; Lutwama, J.; Kohl, A.; Duong, V.; Ponlawat, A.; Sylla, M.; Akorli, J.; Otoo, S.; Lutomiah, J.; Sang, R.; Mutebi, J.-P.; Cao-Lormeau, V.-M.; Jarman, R.; Diagne, C.; Faye, O.; Faye, O.; Sall, A.; McBride, C.; Montagutelli, X.; Rašić, G.; Lambrechts, L. Enhanced Zika virus susceptibility of globally invasive Aedes aegypti populations, Science, Volume 370, pp. 991-996 | DOI

[5] Audsley, M.; Ye, Y.; McGraw, E. The microbiome composition of Aedes aegypti is not critical for Wolbachia-mediated inhibition of dengue virus, PLoS Neglected Tropical Diseases, Volume 11, p. 0005426 | DOI

[6] Bellone, R.; Lechat, P.; Mousson, L.; Gilbart, V.; Piorkowski, G.; Bohers, C.; Merits, A.; Kornobis, E.; Reveillaud, J.; Paupy, C.; Vazeille, M.; Martinet, J.-P.; Madec, Y.; Lamballerie, X.; Dauga, C.; Failloux, A.-B. Climate change and vector-borne diseases: a multi-omics approach of temperature-induced changes in the mosquito, Journal of Travel Medicine, Volume 30 | DOI

[7] Bhatt, S.; Gething, P.; Brady, O.; Messina, J.; Farlow, A.; Moyes, C.; Drake, J.; Brownstein, J.; Hoen, A.; Sankoh, O.; Myers, M.; George, D.; Jaenisch, T.; Wint, G.; Simmons, C.; Scott, T.; Farrar, J.; Hay, S. The global distribution and burden of dengue, Nature, Volume 496, pp. 504-507 | DOI

[8] Bohers, C.; Mousson, L.; Madec, Y.; Vazeille, M.; Rhim, A.; M’ghirbi, Y.; Bouattour, A.; Failloux, A.-B. The recently introduced Aedes albopictus in Tunisia has the potential to transmit chikungunya, dengue and Zika viruses, PLOS Neglected Tropical Diseases, Volume 14, p. 0008475 | DOI

[9] Bonilauri, P.; Bellini, R.; Calzolari, M.; Angelini, R.; Venturi, L.; Fallacara, F.; Cordioli, P.; Angelini, P.; Venturelli, C.; Merialdi, G.; Dottori, M. Chikungunya Virus in Aedes albopictus, Italy, Emerging Infectious Diseases, Volume 14, pp. 852-854 | DOI

[10] Bosio, C.; Beaty, B.; Black, W. Quantitative genetics of vector competence for dengue-2 virus in Aedes aegypti, The American Journal of Tropical Medicine and Hygiene, Volume 59, pp. 965-970 | DOI

[11] Bosio, C.; Fulton, R.; Salasek, M.; Beaty, B.; Black, W. Quantitative trait loci that control vector competence for dengue-2 virus in the mosquito Aedes aegypti, Genetics, Volume 156, pp. 687-698 | DOI

[12] Brinker, P.; Fontaine, M.; Beukeboom, L.; Salles, J. Host, Symbionts, and the Microbiome: The Missing Tripartite Interaction, Trends in Microbiology, Volume 27, pp. 480-488 | DOI

[13] Carpenter, A.; Bryant, W.; Santos, S.; R.J. Infection of Aedes aegypti Mosquitoes with Midgut-Attenuated Sindbis Virus Reduces, but Does Not Eliminate, Disseminated Infection, Journal of Virology, Volume 95, p. 00136-21 | DOI

[14] Chowdhury, A.; Modahl, C.; Missé, D.; Kini, R.; Pompon, J. High resolution proteomics of Aedes aegypti salivary glands infected with either dengue, Zika or chikungunya viruses identify new virus specific and broad antiviral factors, Scientific Reports, Volume 11, p. 23696 | DOI

[15] Christofferson, R.; Chisenhall, D.; Wearing, H.; Mores, C. Chikungunya Viral Fitness Measures within the Vector and Subsequent Transmission Potential, PLoS ONE, Volume 9, p. 110538 | DOI

[16] Christofferson, R.; Mores, C. Estimating the Magnitude and Direction of Altered Arbovirus Transmission Due to Viral Phenotype, PLoS ONE, Volume 6, p. 16298 | DOI

[17] Ciano, K.; Saredy, J.; Bowers, D. Heparan Sulfate Proteoglycan: An Arbovirus Attachment Factor Integral to Mosquito Salivary Gland Ducts, Viruses, Volume 6, pp. 5182-5197 | DOI

[18] Coffey, L.; Failloux, A.-B.; Weaver, S. Chikungunya Virus–Vector Interactions, Viruses, Volume 6, pp. 4628-4663 | DOI

[19] Delatte, H.; Desvars, A.; Bouétard, A.; Bord, S.; Gimonneau, G.; Vourc’h, G.; Fontenille, D. Blood-Feeding Behavior of Aedes albopictus, a Vector of Chikungunya on La Réunion, Vector-Borne and Zoonotic Diseases, Volume 10, pp. 249-258 | DOI

[20] Delisle, E.; Rousseau, C.; Broche, B.; Leparc-Goffart, I.; L’Ambert, G.; Cochet, A.; Prat, C.; Foulongne, V.; Ferré, J.; Catelinois, O.; Flusin, O.; Tchernonog, E.; Moussion, I.; Wiegandt, A.; Septfons, A.; Mendy, A.; Moyano, M.; Laporte, L.; Maurel, J.; Jourdain, F.; Reynes, J.; Paty, M.; Golliot, F. Chikungunya outbreak in Montpellier, France, September to October 2014, Eurosurveillance, Volume 20 | DOI

[21] Dickson, L.; Sanchez-Vargas, I.; Sylla, M.; Fleming, K.; Black, W. Vector competence in West African Aedes aegypti Is Flavivirus species and genotype dependent, PLoS neglected tropical diseases, Volume 8, p. 3153 | DOI

[22] Dong, Y.; Dong, S.; Dizaji, N.; Rutkowski, N.; Pohlenz, T.; Myles, K.; Dimopoulos, G. The Aedes aegypti siRNA pathway mediates broad-spectrum defense against human pathogenic viruses and modulates antibacterial and antifungal defenses, PLoS Biology, Volume 20, p. 3001668 | DOI

[23] Dubrulle, M.; Mousson, L.; Moutailler, S.; Vazeille, M.; Failloux, A.-B. Chikungunya virus and Aedes mosquitoes: saliva is infectious as soon as two days after oral infection, PloS One, Volume 4, p. 5895 | DOI

[24] Duong, V.; Lambrechts, L.; Paul, R.; Ly, S.; Lay, R.; Long, K.; Huy, R.; Tarantola, A.; Scott, T.; Sakuntabhai, A.; Buchy, P. Asymptomatic humans transmit dengue virus to mosquitoes, Proceedings of the National Academy of Sciences, Volume 112, pp. 14688-14693 | DOI

[25] Favier, C.; Schmit, D.; Mller-Graf, C.; Cazelles, B.; Degallier, N.; Mondet, B.; Dubois, M. Influence of spatial heterogeneity on an emerging infectious disease: the case of dengue epidemics, Proceedings of the Royal Society B: Biological Sciences, Volume 272, pp. 1171-1177 | DOI

[26] Fikrig, K.; Harrington, L. Understanding and interpreting mosquito blood feeding studies: the case of Aedes albopictus, Trends in Parasitology, Volume 37, pp. 959-975 | DOI

[27] Fontaine, A.; Lequime, S.; Moltini-Conclois, I.; Jiolle, D.; Leparc-Goffart, I.; Reiner, R.; Lambrechts, L. Epidemiological significance of dengue virus genetic variation in mosquito infection dynamics, PLOS Pathogens, Volume 14, p. 1007187 | DOI

[28] Franke, F.; Giron, S.; Cochet, A.; Jeannin, C.; Leparc-Goffart, I.; de Valk, H.; Jourdain, F.; de Lamballerie, X.; L’Ambert, G.; Paty, M. C. Autochthonous chikungunya and dengue fever outbreak in Mainland France, 2010-2018, European Journal of Public Health, Volume 29 (2019) no. Supplement_4 | DOI

[29] Franz, A.; Kantor, A.; Passarelli, A.; Clem, R. Tissue Barriers to Arbovirus Infection in Mosquitoes, Viruses, Volume 7, pp. 3741-3767 | DOI

[30] Gloria-Soria, A.; Brackney, D.; Armstrong, P. Saliva collection via capillary method may underestimate arboviral transmission by mosquitoes, Parasites Vectors, Volume 15, p. 103 | DOI

[31] Gloria-Soria, A.; Payne, A.; Bialosuknia, S.; Stout, J.; Mathias, N.; Eastwood, G.; Ciota, A.; Kramer, L.; Armstrong, P. Vector Competence of Aedes albopictus Populations from the Northeastern United States for Chikungunya, Dengue, and Zika Viruses, The American Journal of Tropical Medicine and Hygiene | DOI

[32] Gratz, N. Critical review of the vector status of Aedes albopictus, Medical and Veterinary Entomology, Volume 18, pp. 215-227 | DOI

[33] Greiser-Wilke, I.; Moennig, V.; Kaaden, O.-R.; Figueiredo, L. Most Alphaviruses Share a Conserved Epitopic Region on Their Nucleocapsid Protein, Journal of General Virology, Volume 70, pp. 743-748 | DOI

[34] Guégan, M.; Zouache, K.; Démichel, C.; Minard, G.; Tran Van, V.; Potier, P.; Mavingui, P.; Valiente Moro, C. The mosquito holobiont: fresh insight into mosquito-microbiota interactions, Microbiome, Volume 6 (2018) no. 1 | DOI

[35] Heitmann, A.; Jansen, S.; Lühken, R.; Leggewie, M.; Schmidt-Chanasit, J.; Tannich, E. Forced Salivation As a Method to Analyze Vector Competence of Mosquitoes, Journal of Visualized Experiments : JoVE, Volume 57980 | DOI

[36] Houk, E.; Hardy, J.; Presser, S.; Kramer, L. Dissemination Barriers for Western Equine Encephalomyelitis Virus in Culex tarsalis Infected after Ingestion of Low Viral Doses, The American Journal of Tropical Medicine and Hygiene, Volume 30, pp. 190-197 | DOI

[37] Huang, W.; Rodrigues, J.; Bilgo, E.; Tormo, J. R.; Challenger, J. D.; De Cozar-Gallardo, C.; Pérez-Victoria, I.; Reyes, F.; Castañeda-Casado, P.; Gnambani, E. J.; Hien, D. F. d. S.; Konkobo, M.; Urones, B.; Coppens, I.; Mendoza-Losana, A.; Ballell, L.; Diabate, A.; Churcher, T. S.; Jacobs-Lorena, M. Delftia tsuruhatensis TC1 symbiont suppresses malaria transmission by anopheline mosquitoes, Science, Volume 381 (2023) no. 6657, pp. 533-540 | DOI

[38] van den Hurk, A. F.; Hall-Mendelin, S.; Pyke, A. T.; Smith, G. A.; Mackenzie, J. S. Vector Competence of Australian Mosquitoes for Chikungunya Virus, Vector-Borne and Zoonotic Diseases, Volume 10 (2010) no. 5, pp. 489-495 | DOI

[39] Islam, Z.; Bishop, S.; Savill, N.; Rowland, R.; Lunney, J.; Trible, B.; Doeschl-Wilson, A. Quantitative Analysis of Porcine Reproductive and Respiratory Syndrome (PRRS) Viremia Profiles from Experimental Infection: A Statistical Modelling Approach, PLoS ONE, Volume 8, p. 83567 | DOI

[40] Labadie, K.; Larcher, T.; Joubert, C.; Mannioui, A.; Delache, B.; Brochard, P.; Guigand, L.; Dubreil, L.; Lebon, P.; Verrier, B.; de Lamballerie, X.; Suhrbier, A.; Cherel, Y.; Le Grand, R.; Roques, P. Chikungunya disease in nonhuman primates involves long-term viral persistence in macrophages, Journal of Clinical Investigation, Volume 120 (2010) no. 3, pp. 894-906 | DOI

[41] Labeaud, A.; Bashir, F.; King, C. Measuring the burden of arboviral diseases: the spectrum of morbidity and mortality from four prevalent infections, Population Health Metrics, Volume 9, p. 1 | DOI

[42] Lanciotti, R.; Kosoy, O.; Laven, J.; Panella, A.; Velez, J.; Lambert, A.; Campbell, G. Chikungunya virus in US travelers returning from India, 2006, Emerging Infectious Diseases, Volume 13, pp. 764-767 | DOI

[43] Lequime, S.; Bastide, P.; Dellicour, S.; Lemey, P.; Baele, G. nosoi: A stochastic agent-based transmission chain simulation framework in r, Methods in Ecology and Evolution, Volume 11, pp. 1002-1007 | DOI

[44] Lequime, S.; Dehecq, J.-S.; Matheus, S.; de Laval, F.; Almeras, L.; Briolant, S.; Fontaine, A. Modeling intra-mosquito dynamics of Zika virus and its dose-dependence confirms the low epidemic potential of Aedes albopictus, PLOS Pathogens, Volume 16 (2020) no. 12 | DOI

[45] Mariconti, M.; Obadia, T.; Mousson, L.; Malacrida, A.; Gasperi, G.; Failloux, A.-B.; Yen, P.-S. Estimating the risk of arbovirus transmission in Southern Europe using vector competence data, Scientific Reports, Volume 9, p. 17852 | DOI

[46] Marin-Lopez, A.; Jiang, J.; Wang, Y.; Cao, Y.; MacNeil, T.; Hastings, A.; Fikrig, E. Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination, PLoS Neglected Tropical Diseases, Volume 15, p. 0009442 | DOI

[47] Mayton, E.; Hernandez, H.; Vitek, C.; Christofferson, R. A Method for Repeated, Longitudinal Sampling of Individual Aedes aegypti for Transmission Potential of Arboviruses, Insects, Volume 12, p. 292 | DOI

[48] Merkling, S.; Raquin, V.; Dabo, S.; Henrion-Lacritick, A.; Blanc, H.; Moltini-Conclois, I.; Frangeul, L.; Varet, H.; Saleh, M.-C.; Lambrechts, L. Tudor-SN Promotes Early Replication of Dengue Virus in the Aedes aegypti Midgut, iScience, Volume 23, p. 100870 | DOI

[49] Merwaiss, F.; Filomatori, C.; Susuki, Y.; Bardossy, E.; Alvarez, D.; Saleh, M.-C. Chikungunya virus replication rate determines the capacity of crossing tissue barriers in mosquitoes, Journal of Virology, Volume 95 | DOI

[50] Modahl, C.; Chowdhury, A.; Oliveira, F.; Kini, R.; Pompon, J. Salivary gland RNA-seq from arbovirus-infected Aedes aegypti and Aedes albopictus provides insights into virus transmission, Access Microbiology, Volume 1 | DOI

[51] Moloney, R.; Kmush, B.; Rudolph, K.; Cummings, D.; Lessler, J. Incubation Periods of Mosquito-Borne Viral Infections: A Systematic Review, The American Journal of Tropical Medicine and Hygiene, Volume 90, pp. 882-891 | DOI

[52] Mombouli, J.-V.; Bitsindou, P.; Elion, D.; Grolla, A.; Feldmann, H.; Niama, F.; Parra, H.-J.; Munster, V. Chikungunya Virus Infection, Emerging Infectious Diseases, Volume 19, pp. 1542-1543 | DOI

[53] Monteiro, V. V. S.; Navegantes-Lima, K. C.; de Lemos, A. B.; da Silva, G. L.; de Souza Gomes, R.; Reis, J. F.; Rodrigues Junior, L. C.; da Silva, O. S.; Romão, P. R. T.; Monteiro, M. C. Aedes–Chikungunya Virus Interaction: Key Role of Vector Midguts Microbiota and Its Saliva in the Host Infection, Frontiers in Microbiology, Volume 10 (2019) | DOI

[54] Mousson, L.; Zouache, K.; Arias-Goeta, C.; Raquin, V.; Mavingui, P.; Failloux, A.-B. The native Wolbachia symbionts limit transmission of dengue virus in Aedes albopictus, PLoS neglected tropical diseases, Volume 6, p. 1989 | DOI

[55] Moutailler, S. Fill in one gap in our understanding of CHIKV intra-vector dynamics, Peer Community In Infections, Volume 1, p. 100091 | DOI

[56] Moutailler, S.; Barré, H.; Vazeille, M.; Failloux, A. Recently introduced Aedes albopictus in Corsica is competent to Chikungunya virus and in a lesser extent to dengue virus, Tropical Medicine International Health, Volume 14, pp. 1105-1109 | DOI

[57] Mulatier, M.; Boullis, A.; Dollin, C.; Cebrián-Torrejón, G.; Vega-Rúa, A. Chikungunya Virus Infection and Gonotrophic Cycle Shape Aedes aegypti Oviposition Behavior and Preferences, Viruses, Volume 15 (1043.) | DOI

[58] Murdock, C.; Evans, M.; McClanahan, T.; Miazgowicz, K.; Tesla, B. Fine-scale variation in microclimate across an urban landscape shapes variation in mosquito population dynamics and the potential of Aedes albopictus to transmit arboviral disease, PLoS Neglected Tropical Diseases, Volume 11, p. 0005640 | DOI

[59] Nguyet, M.; Duong, T.; Trung, V.; Nguyen, T.; Tran, C.; Long, V.; Dui, L.; Nguyen, H.; Farrar, J.; Holmes, E.; Rabaa, M.; Bryant, J.; Nguyen, T.; Nguyen, H.; Nguyen, L.; Pham, M.; Nguyen, H.; Luong, T.; Wills, B.; Nguyen, C.; Wolbers, M.; Simmons, C. Host and viral features of human dengue cases shape the population of infected and infectious Aedes aegypti mosquitoes, Proceedings of the National Academy of Sciences of the United States of America, Volume 110, pp. 9072-9077 | DOI

[60] Olmo, R. P.; Ferreira, A. G. A.; Izidoro-Toledo, T. C.; Aguiar, E. R. G. R.; de Faria, I. J. S.; de Souza, K. P. R.; Osório, K. P.; Kuhn, L.; Hammann, P.; de Andrade, E. G.; Todjro, Y. M.; Rocha, M. N.; Leite, T. H. J. F.; Amadou, S. C. G.; Armache, J. N.; Paro, S.; de Oliveira, C. D.; Carvalho, F. D.; Moreira, L. A.; Marois, E.; Imler, J.-L.; Marques, J. T. Control of dengue virus in the midgut of Aedes aegypti by ectopic expression of the dsRNA-binding protein Loqs2, Nature Microbiology, Volume 3 (2018) no. 12, pp. 1385-1393 | DOI

[61] Olmo, R. P.; Todjro, Y. M. H.; Aguiar, E. R. G. R.; de Almeida, J. P. P.; Ferreira, F. V.; Armache, J. N.; de Faria, I. J. S.; Ferreira, A. G. A.; Amadou, S. C. G.; Silva, A. T. S.; de Souza, K. P. R.; Vilela, A. P. P.; Babarit, A.; Tan, C. H.; Diallo, M.; Gaye, A.; Paupy, C.; Obame-Nkoghe, J.; Visser, T. M.; Koenraadt, C. J. M.; Wongsokarijo, M. A.; Cruz, A. L. C.; Prieto, M. T.; Parra, M. C. P.; Nogueira, M. L.; Avelino-Silva, V.; Mota, R. N.; Borges, M. A. Z.; Drumond, B. P.; Kroon, E. G.; Recker, M.; Sedda, L.; Marois, E.; Imler, J.-L.; Marques, J. T. Mosquito vector competence for dengue is modulated by insect-specific viruses, Nature Microbiology, Volume 8 (2023) no. 1, pp. 135-149 | DOI

[62] Pagès, F.; Peyrefitte, C.; Mve, M.; Jarjaval, F.; Brisse, S.; Iteman, I.; Gravier, P.; Tolou, H.; Nkoghe, D.; Grandadam, M. Aedes albopictus Mosquito: The Main Vector of the 2007 Chikungunya Outbreak in Gabon, PLoS ONE, Volume 4, p. 4691 | DOI

[63] Panning, M.; Grywna, K.; van Esbroeck, M.; Emmerich, P.; Drosten, C. Chikungunya Fever in Travelers Returning to Europe from the Indian Ocean Region, 2006, Emerging Infectious Diseases, Volume 14 (2008) no. 3, pp. 416-422 | DOI

[64] Paupy, C.; Delatte, H.; Bagny, L.; Corbel, V.; Fontenille, D. Aedes albopictus, an arbovirus vector: from the darkness to the light, Microbes and Infection / Institut Pasteur, Volume 11, pp. 1177-1185 | DOI

[65] Paupy, C.; Kassa, F.; Caron, M.; Nkoghé, D.; Leroy, E. A Chikungunya Outbreak Associated with the Vector Aedes albopictus in Remote Villages of Gabon, Vector-Borne and Zoonotic Diseases, Volume 12, pp. 167-169 | DOI

[66] Peña-García, V.; Luvall, J.; Christofferson, R. Arbovirus Transmission Predictions Are Affected by Both Temperature Data Source and Modeling Methodologies across Cities in Colombia, Microorganisms, Volume 11, p. 1249 | DOI

[67] Pesko, K.; Westbrook, C.; Mores, C.; Lounibos, L.; Reiskind, M. Effects of Infectious Virus Dose and Bloodmeal Delivery Method on Susceptibility of Aedes aegypti and Aedes albopictus to Chikungunya Virus, Journal of Medical Entomology, Volume 46, pp. 395-399 | DOI

[68] Pompon, J.; Manuel, M.; Ng, G.; Wong, B.; Shan, C.; Manokaran, G.; Soto-Acosta, R.; Bradrick, S.; Ooi, E.; Missé, D.; Shi, P.-Y.; Garcia-Blanco, M. Dengue subgenomic flaviviral RNA disrupts immunity in mosquito salivary glands to increase virus transmission, PLOS Pathogens, Volume 13, p. 1006535 | DOI

[69] Raquin, V.; Lambrechts, L. Dengue virus replicates and accumulates in Aedes aegypti salivary glands, Virology, Volume 507, pp. 75-81 | DOI

[70] Raquin, V.; Merkling, S.; Gausson, V.; Moltini-Conclois, I.; Frangeul, L.; Varet, H.; Dillies, M.-A.; Saleh, M.-C.; Lambrechts, L. Individual co-variation between viral RNA load and gene expression reveals novel host factors during early dengue virus infection of the Aedes aegypti midgut, PLoS Neglected Tropical Diseases, Volume 11, p. 0006152 | DOI

[71] Raquin, V.; Moro, C.; Saucereau, Y.; Tran, F.-H.; Potier, P.; Mavingui, P. Native Wolbachia from Aedes albopictus Blocks Chikungunya Virus Infection In Cellulo, PLOS ONE, Volume 10, p. 0125066 | DOI

[72] R Core Team R: A Language and Environment for Statistical Computing,

[73] Riswari, S.; Ma’roef, C.; Djauhari, H.; Kosasih, H.; Perkasa, A.; Yudhaputri, F.; Artika, I.; Williams, M.; van der Ven, A.; Myint, K.; Alisjahbana, B.; Ledermann, J.; Powers, A.; Jaya, U. Study of viremic profile in febrile specimens of chikungunya in Bandung, Indonesia, Journal of Clinical Virology, Volume 74 (2016), pp. 61-65 | DOI

[74] Robison, A.; Young, M.; Byas, A.; Rückert, C.; Ebel, G. Comparison of Chikungunya Virus and Zika Virus Replication and Transmission Dynamics in Aedes aegypti Mosquitoes, The American journal of tropical medicine and hygiene | DOI

[75] Sanchez-Vargas, I.; Harrington, L.; Black, W.; Olson, K. Analysis of Salivary Glands and Saliva from Aedes albopictus and Aedes aegypti Infected with Chikungunya Viruses, Insects, Volume 10, p. 39 | DOI

[76] Sanchez-Vargas, I.; Olson, K.; Black, W. The Genetic Basis for Salivary Gland Barriers to Arboviral Transmission, Insects, Volume 12, p. 73 | DOI

[77] Schuffenecker, I.; Iteman, I.; Michault, A.; Murri, S.; Frangeul, L.; Vaney, M.-C.; Lavenir, R.; Pardigon, N.; Reynes, J.-M.; Pettinelli, F.; Biscornet, L.; Diancourt, L.; Michel, S.; Duquerroy, S.; Guigon, G.; Frenkiel, M.-P.; Bréhin, A.-C.; Cubito, N.; Desprès, P.; Kunst, F.; Rey, F.; Zeller, H.; Brisse, S. Genome Microevolution of Chikungunya Viruses Causing the Indian Ocean Outbreak, PLoS Medicine, Volume 3, p. 263 | DOI

[78] Schwartz, O.; Albert, M. Biology and pathogenesis of chikungunya virus, Nature Reviews Microbiology, Volume 8, pp. 491-500 | DOI

[79] Shocket, M.; Verwillow, A.; Numazu, M.; Slamani, H.; Cohen, J.; Moustaid, F.; Rohr, J.; Johnson, L.; Mordecai, E. Transmission of West Nile and five other temperate mosquito-borne viruses peaks at temperatures between 23°C and 26°C, eLife, Volume 9, p. 58511 | DOI

[80] Smith, D. L.; Battle, K. E.; Hay, S. I.; Barker, C. M.; Scott, T. W.; McKenzie, F. E. Ross, Macdonald, and a Theory for the Dynamics and Control of Mosquito-Transmitted Pathogens, PLoS Pathogens, Volume 8 (2012) no. 4 | DOI

[81] Tsetsarkin, K.; Vanlandingham, D.; McGee, C.; Higgs, S. A single mutation in chikungunya virus affects vector specificity and epidemic potential, PLoS pathogens, Volume 3, p. 201 | DOI

[82] Vásquez, V.; Kueppers, L.; Rašić, G.; Marshall, J. wMel replacement of dengue-competent mosquitoes is robust to near-term climate change, Nature Climate Change, Volume 13, pp. 848-855 | DOI

[83] Vazeille, M.; Madec, Y.; Mousson, L.; Bellone, R.; Barré-Cardi, H.; Sousa, C. A.; Jiolle, D.; Yébakima, A.; de Lamballerie, X.; Failloux, A.-B. Zika virus threshold determines transmission by European Aedes albopictus mosquitoes, Emerging Microbes and Infections, Volume 8 (2019) no. 1, pp. 1668-1678 | DOI

[84] Vazeille, M.; Moutailler, S.; Coudrier, D.; Rousseaux, C.; Khun, H.; Huerre, M.; Thiria, J.; Dehecq, J.-S.; Fontenille, D.; Schuffenecker, I.; Despres, P.; Failloux, A.-B. Two Chikungunya Isolates from the Outbreak of La Reunion (Indian Ocean) Exhibit Different Patterns of Infection in the Mosquito, Aedes albopictus. PLoS ONE, 2, p. 1168 | DOI

[85] Vega-Rúa, A.; Lourenço-de-Oliveira, R.; Mousson, L.; Vazeille, M.; Fuchs, S.; Yébakima, A.; Gustave, J.; Girod, R.; Dusfour, I.; Leparc-Goffart, I.; Vanlandingham, D. L.; Huang, Y.-J. S.; Lounibos, L. P.; Mohamed Ali, S.; Nougairede, A.; de Lamballerie, X.; Failloux, A.-B. Chikungunya Virus Transmission Potential by Local Aedes Mosquitoes in the Americas and Europe, PLOS Neglected Tropical Diseases, Volume 9 (2015) no. 5 | DOI

[86] Vega-Rúa, A.; Marconcini, M.; Madec, Y.; Manni, M.; Carraretto, D.; Gomulski, L.; Gasperi, G.; Failloux, A.-B.; Malacrida, A. Vector competence of Aedes albopictus populations for chikungunya virus is shaped by their demographic history, Communications Biology, Volume 3, p. 326 | DOI

[87] Vega-Rúa, A.; Schmitt, C.; Bonne, I.; Locker, J.; Failloux, A.-B. Chikungunya Virus Replication in Salivary Glands of the Mosquito Aedes albopictus, Viruses, Volume 7, pp. 5902-5907 | DOI

[88] Vega-Rua, A.; Zouache, K.; Caro, V.; Diancourt, L.; Delaunay, P.; Grandadam, M.; Failloux, A.-B. High Efficiency of Temperate Aedes albopictus to Transmit Chikungunya and Dengue Viruses in the Southeast of France, PLoS ONE, Volume 8, p. 59716 | DOI

[89] Venturi, G.; Luca, M.; Fortuna, C.; Remoli, M.; Riccardo, F.; Severini, F.; Toma, L.; Manso, M.; Benedetti, E.; Caporali, M.; Amendola, A.; Fiorentini, C.; Liberato, C.; Giammattei, R.; Romi, R.; Pezzotti, P.; Rezza, G.; Rizzo, C. Detection of a chikungunya outbreak in Central Italy, August to September 2017, Eurosurveillance, Volume 22, pp. 17-00646 | DOI

[90] Viglietta, M.; Bellone, R.; Blisnick, A.; Failloux, A.-B. Vector Specificity of Arbovirus Transmission, Frontiers in Microbiology, Volume 12, p. 773211 | DOI

[91] Viginier, B.; Cappuccio, L.; Garnier, C.; Martin, E.; Maisse, C.; Moro, C.; Minard, G.; Fontaine, A.; Lequime, S.; Ratinier, M.; Arnaud, F.; Raquin, V. Chikungunya intra-vector infection dynamics in a French Aedes albopictus population reveals low vector barrier intensity and supports an explosive epidemic potential | DOI

[92] Wagar, Z.; Tree, M.; Mpoy, M.; Conway, M. Low density lipopolyprotein inhibits flavivirus acquisition in Aedes aegypti, Insect Molecular Biology, Volume 26, pp. 734-742 | DOI

[93] Wickham, H.; Averick, M.; Bryan, J.; Chang, W.; McGowan, L.; François, R.; Grolemund, G.; Hayes, A.; Henry, L.; Hester, J.; Kuhn, M.; Pedersen, T.; Miller, E.; Bache, S.; Müller, K.; Ooms, J.; Robinson, D.; Seidel, D.; Spinu, V.; Takahashi, K.; Vaughan, D.; Wilke, C.; Woo, K.; Yutani, H. Welcome to the Tidyverse, Journal of Open Source Software, Volume 4 | DOI

[94] Williams, A.; Franz, A.; Reid, W.; Olson, K. Antiviral Effectors and Gene Drive Strategies for Mosquito Population Suppression or Replacement to Mitigate Arbovirus Transmission by Aedes aegypti, Insects, Volume 11, p. 52 | DOI

[95] Wimberly, M.; Davis, J.; Evans, M.; Hess, A.; Newberry, P.; Solano-Asamoah, N.; Murdock, C. Land cover affects microclimate and temperature suitability for arbovirus transmission in an urban landscape, PLoS Neglected Tropical Diseases, Volume 14, p. 0008614 | DOI

[96] Zhang, Y.; Yan, H.; Li, X.; Zhou, D.; Zhong, M.; Yang, J.; Zhao, B.; Fan, X.; Fan, J.; Shu, J.; Lu, M.; Jin, X.; Zhang, E.; Yan, H. A high-dose inoculum size results in persistent viral infection and arthritis in mice infected with chikungunya virus, PLoS Neglected Tropical Diseases, Volume 16, p. 0010149 | DOI

[97] Zhu, Y.; Zhang, R.; Zhang, B.; Zhao, T.; Wang, P.; Liang, G.; Cheng, G. Blood meal acquisition enhances arbovirus replication in mosquitoes through activation of the GABAergic system, Nature Communications, Volume 8, p. 1262 | DOI

[98] Zouache, K.; Fontaine, A.; Vega-Rua, A.; Mousson, L.; Thiberge, J.-M.; Lourenco-De-Oliveira, R.; Caro, V.; Lambrechts, L.; Failloux, A.-B. Three-way interactions between mosquito population, viral strain and temperature underlying chikungunya virus transmission potential, Proceedings of the Royal Society B: Biological Sciences, Volume 281, p. 20141078 | DOI

[99] Zouache, K.; Michelland, R.; Failloux, A.-B.; Grundmann, G.; Mavingui, P. Chikungunya virus impacts the diversity of symbiotic bacteria in mosquito vector, Molecular Ecology, Volume 21, pp. 2297-2309 | DOI

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