Section: Infections
Topic: Biology of interactions, Microbiology, Ecology

Influence of endosymbionts on the reproductive fitness of the tick Ornithodoros moubata

10.24072/pcjournal.334 - Peer Community Journal, Volume 3 (2023), article no. e102.

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Over the past decade, many studies have demonstrated the crucial role of the tick microbiome in tick biology. The soft tick Ornithodoros moubata is a hematophagous ectoparasite of Suidae, best known for transmitting the  African swine fever virus. Its bacterial microbiota is characterized by a high prevalence of Francisella-like and Rickettsia endosymbionts. The present study aims to better understand the potential influence of these two major members of the tick microbiota on the reproductive fitness of O. moubata. A total of 132 adult female ticks were treated with antibiotics using gentamycin or rifampicin added to the blood meal. Half of the ticks were also supplemented with B vitamins to address the nutritional role of endosymbionts. Over two periods of 50 days, several traits related to the reproductive fitness were monitored to investigate the importance of Francisella and Rickettsia for these traits. It appeared that most of the reproductive parameters considered were not affected. However, antibiotic treatments induced an increase in the tick survival, indicating a potential fitness cost of harboring endosymbionts during the tick reproductive cycle. Similarly, 366 first stage nymphs of O. moubata were exposed to the same treatments for molecular quantification of both endosymbionts. Results from qPCR suggested that the treatments had a bacteriostatic effect on endosymbionts without completely eliminating neither Francisella-like endosymbiont nor Rickettsia.

Published online:
DOI: 10.24072/pcjournal.334
Type: Research article
Keywords: Soft tick; Ornithodoros; microbiota; antibiotic treatment; Francisella-like endosymbiont; Rickettsia
Taraveau, Florian 1; Pollet, Thomas 1; Duhayon, Maxime 1; Gardès, Laëtitia 1; Jourdan-Pineau, Hélène 1

1 UMR ASTRE, CIRAD, INRAE, Campus de Baillarguet, F-34398 Montpellier, France
License: CC-BY 4.0
Copyrights: The authors retain unrestricted copyrights and publishing rights
     author = {Taraveau, Florian and Pollet, Thomas and Duhayon, Maxime and Gard\`es, La\"etitia and Jourdan-Pineau, H\'el\`ene},
     title = {Influence of endosymbionts on the reproductive fitness of the tick {\protect\emph{Ornithodoros} moubata}},
     journal = {Peer Community Journal},
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Taraveau, Florian; Pollet, Thomas; Duhayon, Maxime; Gardès, Laëtitia; Jourdan-Pineau, Hélène. Influence of endosymbionts on the reproductive fitness of the tick Ornithodoros moubata. Peer Community Journal, Volume 3 (2023), article  no. e102. doi : 10.24072/pcjournal.334.

PCI peer reviews and recommendation, and links to data, scripts, code and supplementary information: 10.24072/pci.infections.100087

Conflict of interest of the recommender and peer reviewers:
The recommender in charge of the evaluation of the article and the reviewers declared that they have no conflict of interest (as defined in the code of conduct of PCI) with the authors or with the content of the article.

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