A meta-analysis of the effect of protein synthesis inhibitors on rodent fear conditioning

10.24072/pcjournal.339 - Peer Community Journal, Volume 3 (2023), article no. e110.

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Systematic reviews and meta-analyses have been increasingly recognized for their potential value in pre-clinical research, but their applications have not been extensively explored in behavioral neuroscience. In this work, we studied protein synthesis inhibition, a classic intervention used to disrupt fear learning, reconsolidation, and extinction in rodents, to explore how meta-analyses can identify potential moderators of its effect. We initially performed separate meta-analyses for different injection sites and target sessions to evaluate the effect of the intervention in various scenarios. Heterogeneity was further investigated by multilevel meta-regression models aggregating various sites, with article or research group as additional levels. We detected robust effects of protein synthesis inhibitors on training and reconsolidation, but not on extinction, possibly due to the lower number of studies on the latter. Our analyses identified some well-established moderators, such as intervention timing and reexposure duration. However, other factors proposed as boundary conditions for reconsolidation, such as memory age and training strength, were not associated with effect size. While our results point to the value of meta-analyses in consolidating findings from the literature, we believe that associations suggested by data synthesis should ideally be verified by well-powered, rigorous confirmatory experiments.

Published online:
DOI: 10.24072/pcjournal.339
Keywords: fear conditioning; preclinical meta-analysis; reproducibility;
Carneiro, Clarissa F. D. 1, 2; Amorim, Felippe E. 1; Amaral, Olavo B. 1

1 Institute of Medical Biochemistry Leopoldo de Meis, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
2 Berlin Institute of Health (BIH) at Charité, BIH QUEST Center for Responsible Research, Berlin, Germany
License: CC-BY 4.0
Copyrights: The authors retain unrestricted copyrights and publishing rights
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Carneiro, Clarissa F. D.; Amorim, Felippe E.; Amaral, Olavo B. A meta-analysis of the effect of protein synthesis inhibitors on rodent fear conditioning. Peer Community Journal, Volume 3 (2023), article  no. e110. doi : 10.24072/pcjournal.339.

Peer reviewed and recommended by PCI : 10.24072/pci.neuro.100144

Conflict of interest of the recommender and peer reviewers:
The recommender in charge of the evaluation of the article and the reviewers declared that they have no conflict of interest (as defined in the code of conduct of PCI) with the authors or with the content of the article.

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