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Section: Evolutionary Biology
Topic: Ecology, Evolution, Genetics/genomics

Genomic relationships among diploid and polyploid species of the genus Ludwigia L. section Jussiaea using a combination of molecular cytogenetic, morphological, and crossing investigations

Barloy, Dominique1  ; Portillo-Lemus, Luis1 ; Krueger-Hadfield, Stacy2 ; Huteau, Virginie3; Coriton, Olivier3 
1 DECOD (Ecosystem Dynamics and Sustainability), Institut Agro, INRAE, IFREMER 35042 Rennes, France
2 Virginia Institute of Marine Science Eastern Shore Laboratory, Wachapreague, VA, 23480, USA
3 Molecular Cytogenetics Platform, IGEPP (Institute for Genetics, Environment and Plant Protection), INRAE, Institut Agro, Univ Rennes, 35653, Le Rheu, France

10.24072/pcjournal.364 - Peer Community Journal, Volume 4 (2024), article no. e8.

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The genus Ludwigia L. section Jussiaea is composed of a polyploid species complex with 2x, 4x, 6x and 10x ploidy levels, suggesting possible hybrid origins. The aim of the present study is to understand the genomic relationships among diploid and polyploid species in the section Jussiaea. Morphological and cytogenetic observations, controlled crosses, genomic in situ hybridization (GISH), and flow cytometry were used to characterize species, ploidy levels, ploidy patterns, and genomic composition across taxa. Genome sizes obtained were in agreement with the diploid, tetraploid, hexaploid, and decaploid ploidy levels. Results of GISH showed that progenitors of Ludwigia stolonifera (4x) were Ludwigia peploides subsp. montevidensis (2x) and Ludwigia helminthorrhiza (2x), which also participated for one part (2x) to the Ludwigia ascendens genome (4x). Ludwigia grandiflora subsp. hexapetala (10x) resulted from the hybridization between L. stolonifera (4x) and Ludwigia grandiflora subsp. grandiflora (6x). One progenitor of L. grandiflora subsp. grandiflora was identified as L. peploides (2x). Our results suggest the existence of several processes of hybridization, leading to polyploidy, and possibly allopolyploidy, in the section Jussiaea due to the diversity of ploidy levels. The success of GISH opens up the potential for future studies to identify other missing progenitors in Ludwigia L. as well as other taxa.

Published online:
DOI: 10.24072/pcjournal.364
Type: Research article
Keywords: GISH, invasive plant, Ludwigia L., Onagraceae, polyploidy, phylogenetics
Barloy, Dominique 1; Portillo-Lemus, Luis 1; Krueger-Hadfield, Stacy 2; Huteau, Virginie 3; Coriton, Olivier 3

1 DECOD (Ecosystem Dynamics and Sustainability), Institut Agro, INRAE, IFREMER 35042 Rennes, France
2 Virginia Institute of Marine Science Eastern Shore Laboratory, Wachapreague, VA, 23480, USA
3 Molecular Cytogenetics Platform, IGEPP (Institute for Genetics, Environment and Plant Protection), INRAE, Institut Agro, Univ Rennes, 35653, Le Rheu, France
License: CC-BY 4.0
Copyrights: The authors retain unrestricted copyrights and publishing rights 
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     title = {Genomic relationships among\protect\textsuperscript{ }diploid and polyploid species of the genus {\protect\emph{Ludwigia}} {L.} section {\protect\emph{Jussiaea}} using a combination of molecular cytogenetic, morphological, and crossing investigations},
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Barloy, Dominique; Portillo-Lemus, Luis; Krueger-Hadfield, Stacy; Huteau, Virginie; Coriton, Olivier. Genomic relationships among diploid and polyploid species of the genus Ludwigia L. section Jussiaea using a combination of molecular cytogenetic, morphological, and crossing investigations. Peer Community Journal, Volume 4 (2024), article  no. e8. doi : 10.24072/pcjournal.364. https://peercommunityjournal.org/articles/10.24072/pcjournal.364/

Peer reviewed and recommended by PCI : 10.24072/pci.evolbiol.100645

Conflict of interest of the recommender and peer reviewers:
The recommender in charge of the evaluation of the article and the reviewers declared that they have no conflict of interest (as defined in the code of conduct of PCI) with the authors or with the content of the article.

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