Section: Zoology
Topic: Population biology

Up and to the light: intra- and interspecific variability of photo- and geo-tactic oviposition preferences in genus Trichogramma

10.24072/pcjournal.78 - Peer Community Journal, Volume 2 (2022), article no. e3.

Get full text PDF Peer reviewed and recommended by PCI

Trichogramma are parasitic microwasps much used as biological control agents. The genus is known to harbor tremendous diversity, at both inter- and intra-specific levels. The successful selection of Trichogramma strains for biocontrol depends on characterizing the existing diversity in this group, especially regarding oviposition performance and behavior. Oviposition preferences in relation to environmental cues such as light and gravity have received little attention so far, although they are suspected to play an important role in microhabitat selection and parasitism patterns. The extent of their variability, and their potential correlated variations, is virtually unknown. Here we use a novel experimental approach relying on automatic image analysis to characterize the oviposition preferences in relation to light and gravity, as well as their interaction, in 25 populations of Trichogramma from five species. We show that most Trichogramma populations and species harbour preferences for light and preferences for elevated parts. However, the two traits harbor significant inter and intraspecific variation. The effects of light and gravity on oviposition patterns were found to be almost perfectly additive overall, with two exceptions. Oviposition preference patterns were not static but very plastic in time: preferences tended to relax over consecutive days, and the strongest preferences relaxed the fastest, presumably because of the density-dependent effect of resource depletion. A correlation of oviposition patterns with the vegetation stratum at which populations were sampled suggests that different species/populations may be associated with different strata with corresponding differentiation in light- and gravity-related oviposition preferences.

Published online:
DOI: 10.24072/pcjournal.78
Type: Research article
Burte, Victor 1; Perez, Guy 1; Ayed, Faten 1; Groussier, Géraldine 1; Mailleret, Ludovic 1; van Oudenhove, Louise 1; Calcagno, Vincent 1

1 Université Côte d'Azur - CNRS - INRAE, Institut Sophia Agrobiotech, Sophia Antipolis, France
License: CC-BY 4.0
Copyrights: The authors retain unrestricted copyrights and publishing rights
     author = {Burte, Victor and Perez, Guy and Ayed, Faten and Groussier, G\'eraldine and Mailleret, Ludovic and van Oudenhove, Louise and Calcagno, Vincent},
     title = {Up and to the light: intra- and interspecific variability of photo- and geo-tactic oviposition preferences in genus {\protect\emph{Trichogramma}}},
     journal = {Peer Community Journal},
     eid = {e3},
     publisher = {Peer Community In},
     volume = {2},
     year = {2022},
     doi = {10.24072/pcjournal.78},
     url = {}
AU  - Burte, Victor
AU  - Perez, Guy
AU  - Ayed, Faten
AU  - Groussier, Géraldine
AU  - Mailleret, Ludovic
AU  - van Oudenhove, Louise
AU  - Calcagno, Vincent
TI  - Up and to the light: intra- and interspecific variability of photo- and geo-tactic oviposition preferences in genus Trichogramma
JO  - Peer Community Journal
PY  - 2022
VL  - 2
PB  - Peer Community In
UR  -
DO  - 10.24072/pcjournal.78
ID  - 10_24072_pcjournal_78
ER  - 
%0 Journal Article
%A Burte, Victor
%A Perez, Guy
%A Ayed, Faten
%A Groussier, Géraldine
%A Mailleret, Ludovic
%A van Oudenhove, Louise
%A Calcagno, Vincent
%T Up and to the light: intra- and interspecific variability of photo- and geo-tactic oviposition preferences in genus Trichogramma
%J Peer Community Journal
%D 2022
%V 2
%I Peer Community In
%R 10.24072/pcjournal.78
%F 10_24072_pcjournal_78
Burte, Victor; Perez, Guy; Ayed, Faten; Groussier, Géraldine; Mailleret, Ludovic; van Oudenhove, Louise; Calcagno, Vincent. Up and to the light: intra- and interspecific variability of photo- and geo-tactic oviposition preferences in genus Trichogramma. Peer Community Journal, Volume 2 (2022), article  no. e3. doi : 10.24072/pcjournal.78.

Peer reviewed and recommended by PCI : 10.24072/pci.zool.100008

Conflict of interest of the recommender and peer reviewers:
The recommender in charge of the evaluation of the article and the reviewers declared that they have no conflict of interest (as defined in the code of conduct of PCI) with the authors or with the content of the article.

[1] Ambrosius, F.; Adler, C.; Reichmuth, C.; Steidle, J. L. M. Invasion of Trichogramma evanescens into food packages and the risk of food contamination. Implementation of biocontrol in practice in temperate regions–present and near future., DIAS report, Volume 119 (2005), pp. 109-118

[2] Bigler, F.; Bieri, M.; Fritschy, A.; Seidel, K. Variation in locomotion between laboratory strains of Trichogramma maidis and its impact on parasitism of eggs of Ostrinia nubilalis in the field, Entomologia Experimentalis et Applicata, Volume 49 (1988) no. 3, pp. 283-290 | DOI

[3] Brodeur, J.; McNeil, J. N. Overwintering microhabitat selection by an Endoparasitoid (Hymenoptera: Aphidiidae): Induced phototactic and thigmokinetic responses in dying hosts, Journal of Insect Behavior, Volume 3 (1990) no. 6, pp. 751-763 | DOI

[4] Brower, J. H.; Cline, L. D. Response of Trichogramma pretiosum and T. evanescens to Whitelight, Blacklight or No-Light Suction Traps, The Florida Entomologist, Volume 67 (1984) no. 2, pp. 262-268 | DOI

[5] Bruins, E. B. A. W.; Wajnberg, E.; Pak, G. A. Genetic variability in the reactive distance in Trichogramma brassicae after automatic tracking of the walking path, Entomologia Experimentalis et Applicata, Volume 72 (1994) no. 3, pp. 297-303 | DOI

[6] Bruno, J. F.; Cardinale, B. J. Cascading effects of predator richness, Frontiers in Ecology and the Environment, Volume 6 (2008) no. 10, pp. 539-546 | DOI

[7] Calcagno, V.; Bonhomme, V.; Thomas, Y.; Singer, M. C.; Bourguet, D. Divergence in behaviour between the European corn borer, Ostrinia nubilalis , and its sibling species Ostrinia scapulalis : adaptation to human harvesting?, Proceedings of the Royal Society B: Biological Sciences, Volume 277 (2010) no. 1694, pp. 2703-2709 | DOI

[8] Consoli FL,; Parra JR,; Zucchi RA Egg Parasitoids in Agroecosystems with Emphasis on Trichogramma, Springer Netherlands, Dordrecht, (2010) | DOI

[9] Conti E; Bin F Parasitoids of concealed noctuid eggs and their potential in biological control of Gramineae stemborers, Redia, Volume 83 (2000), pp. 87-104

[10] Dahirel, M.; Bertin, A.; Haond, M.; Blin, A.; Lombaert, E.; Calcagno, V.; Fellous, S.; Mailleret, L.; Malausa, T.; Vercken, E. Shifts from pulled to pushed range expansions caused by reduction of landscape connectivity, Oikos, Volume 130 (2021) no. 5, pp. 708-724 | DOI

[11] Gariepy, V.; Boivin, G.; Brodeur, J. Why two species of parasitoids showed promise in the laboratory but failed to control the soybean aphid under field conditions, Biological Control, Volume 80 (2015), pp. 1-7 | DOI

[12] Van Atta, K. J.; Potter, K. A.; Woods, H. A. Effects of UV-B on Environmental Preference and Egg Parasitization byTrichogrammaWasps (Hymenoptera: Trichogrammatidae), Journal of Entomological Science, Volume 50 (2015) no. 4, pp. 318-325 | DOI

[13] Fournier, F.; Pelletier, D.; Vigneault, C.; Goyette, B.; Boivin, G. Effect of Barometric Pressure on Flight Initiation by <I>Trichogramma pretiosum</I> and <I>Trichogramma evanescens</I> (Hymenoptera: Trichogrammatidae), Environmental Entomology, Volume 34 (2005) no. 6, pp. 1534-1540 | DOI

[14] Gardner, J.; Hoffmann, M. P. How important is vision in short-range host-finding by Trichogramma ostriniae used for augmentative biological control?, Biocontrol Science and Technology, Volume 30 (2020) no. 6, pp. 531-547 | DOI

[15] Henderson, M. M.; Gardner, J.; Raguso, R. A.; Hoffmann, M. P. Trichogramma ostriniae (Hymenoptera: Trichogrammatidae) response to relative humidity with and without host cues, Biocontrol Science and Technology, Volume 27 (2017) no. 1, pp. 128-138 | DOI

[16] Honda, J. Y.; Silva, I. M.; Vereijssen, J.; Stouthamer, R. Laboratory bioassay and greenhouse evaluation of Trichogramma cordubensis strains from Portugal, BioControl, Volume 44 (1999) no. 1, pp. 1-11 | DOI

[17] Hunt, R. E.; Nault, L. R. Roles of interplant movement, acoustic communication, and phototaxis in mate-location behavior of the leafhopper Graminella nigrifrons, Behavioral Ecology and Sociobiology, Volume 28 (1991) no. 5, pp. 315-320 | DOI

[18] Hussein, M. Y.; Kameldeer, A. K. A field study on the oviposition ofOstrinia furnacalisGuenee (Lepidoptera: Pyralidae) on maize in Selangor, Malaysia, Tropical Pest Management, Volume 34 (1988) no. 1, pp. 44-47 | DOI

[19] Grieshop, M. J.; Flinn, P. W.; Nechols, J. R.; Scholler, M. Host-Foraging Success of Three Species of Trichogramma (Hymenoptera: Trichogrammatidae) in a Simulated Retail Environment, Journal of Economic Entomology, Volume 100 (2014) no. 2, pp. 591-598 | DOI

[20] Kering, K. K.; Kibii, B. J.; Wei, H. Biocontrol of phytobacteria with bacteriophage cocktails, Pest Management Science, Volume 75 (2019) no. 7, pp. 1775-1781 | DOI

[21] Kölliker-Ott, U. M.; Bigler, F.; Hoffmann, A. A. Field dispersal and host location of Trichogramma brassicae is influenced by wing size but not wing shape, Biological Control, Volume 31 (2004) no. 1, pp. 1-10 | DOI

[22] Lartigue, S.; Yalaoui, M.; Belliard, J.; Caravel, C.; Jeandroz, L.; Groussier, G.; Calcagno, V.; Louâpre, P.; Dechaume‐Moncharmont, F.; Malausa, T.; Moreau, J. Consistent variations in personality traits and their potential for genetic improvement of biocontrol agents: Trichogramma evanescens as a case study, Evolutionary Applications (2021) | DOI

[23] Perez G; Burte V; Baron O; Calcagno V, Cahiers Techniques de l’INRA; numéro spécial Entomologie, Volume Chapitre 3, (2017), pp. 135-142

[24] Powers, S. M.; Hampton, S. E. Open science, reproducibility, and transparency in ecology, Ecological Applications, Volume 29 (2018) no. 1 | DOI

[25] R Core Team R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. URL, (2020)

[26] Ris, N.; Groussier, G.; Sellier, N.; Marchand A; Warot, S. BRC “Egg Parasitoids Collection” (EP-Coll),, Portail Data INRAE, V1, (2018)

[27] Schindelin, J.; Arganda-Carreras, I.; Frise, E.; Kaynig, V.; Longair, M.; Pietzsch, T.; Preibisch, S.; Rueden, C.; Saalfeld, S.; Schmid, B.; Tinevez, J.-Y.; White, D. J.; Hartenstein, V.; Eliceiri, K.; Tomancak, P.; Cardona, A. Fiji: an open-source platform for biological-image analysis, Nature Methods, Volume 9 (2012) no. 7, pp. 676-682 | DOI

[28] Schmitz, O. J. Predator Diversity And Trophic Interactions, Ecology, Volume 88 (2007) no. 10, pp. 2415-2426 | DOI

[29] Singer, M. C. Adaptive and maladaptive consequences of “matching habitat choice:” lessons from a rapidly-evolving butterfly metapopulation, Evolutionary Ecology, Volume 29 (2015) no. 6, pp. 905-925 | DOI

[30] Spangler, S. M.; Calvin, D. D. Vertical Distribution of European Corn Borer (Lepidoptera: Crambidae) Egg Masses on Sweet Corn, Environmental Entomology, Volume 30 (2001) no. 2, pp. 274-279 | DOI

[31] Thorpe, K. W. Effects of height and habitat type on egg parasitism by Trichogramma minutum and T. pretiosum (Hymenoptera: Trichogrammatidae), Agriculture, Ecosystems & Environment, Volume 12 (1985) no. 2, pp. 117-126 | DOI

[32] Thorpe, K. W.; Dively, G. P. Effects of Arena Size on Laboratory Evaluations of the Egg Parasitoids Trichogramma minutum, T. pretiosum, and T. exiguum (Hymenoptera: Trichogrammatidae), Environmental Entomology, Volume 14 (1985) no. 6, pp. 762-767 | DOI

[33] Lenteren, J. C. v.; Hale, A.; Klapwijk, J. N.; Schelt, J. v.; Steinberg, S. Guidelines for quality control of commercially produced natural enemies., Quality control and production of biological control agents: theory and testing procedures, CABI, Wallingford, 2003, pp. 265-303 | DOI

[34] Wajnberg, E.; Fauvergue, X.; Pons, O. Patch leaving decision rules and the Marginal Value Theorem: an experimental analysis and a simulation model, Behavioral Ecology, Volume 11 (2000) no. 6, pp. 577-586 | DOI

[35] Zhang, Y.; Wang, X.-X.; Jing, X.; Tian, H.-G.; Liu, T.-X. Winged Pea Aphids Can Modify Phototaxis in Different Development Stages to Assist Their Host Distribution, Frontiers in Physiology, Volume 7 (2016) | DOI

Cited by Sources: