Section: Infections
Topic: Biology of interactions, Microbiology, Health sciences

Chikungunya intra-vector dynamics in Aedes albopictus from Lyon (France) upon exposure to a human viremia-like dose range reveals vector barrier’s permissiveness and supports local epidemic potential

Viginier, Barbara1 ; Cappuccio, Lucie1 ; Garnier, Céline1; Martin, Edwige2; Maisse, Carine1 ; Valiente Moro, Claire2 ; Minard, Guillaume2 ; Fontaine, Albin3, 4, 5 ; Lequime, Sébastian6 ; Ratinier, Maxime1 ; Arnaud, Frédérick1 ; Raquin, Vincent1  
1 IVPC UMR754, INRAE, Universite Claude Bernard Lyon 1, EPHE, PSL Research University, F-69007 Lyon, France
2 Universite Claude Bernard Lyon 1, CNRS, INRAE, VetAgro Sup, UMR Ecologie Microbienne, F-69622 Villeurbanne, France
3 Unité Parasitologie et Entomologie, Département Microbiologie et maladies infectieuses, Institut de Recherche Biomédicale des Armées (IRBA), Marseille, France
4 Aix Marseille Univ, IRD, SSA, AP-HM, UMR Vecteurs–Infections Tropicales et Méditerranéennes (VITROME), Marseille, France
5 IHU Méditerranée Infection, Marseille, France
6 Cluster of Microbial Ecology, Groningen Institute for Evolutionary Life Sciences, University of Groningen, Groningen, The Netherlands

Corresponding author(s): Raquin, Vincent (vincent.raquin@ephe.psl.eu)

10.24072/pcjournal.326 - Peer Community Journal, Volume 3 (2023), article no. e96.

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Arbovirus emergence and epidemic potential, as approximated by the vectorial capacity formula, depends on host and vector parameters, including the vector’s intrinsic ability to replicate then transmit the pathogen known as vector competence. Vector competence is a complex, time-dependent, quantitative phenotype influenced by biotic and abiotic factors. A combination of experimental and modelling approaches is required to assess arbovirus intra-vector dynamics and estimate epidemic potential. In this study, we measured infection, dissemination, and transmission dynamics of chikungunya virus (CHIKV) in a field-derived Aedes albopictus population (Lyon, France) after oral exposure to a range of virus doses spanning human viraemia. Statistical modelling indicates rapid and efficient CHIKV progression in the vector mainly due to an absence of a dissemination barrier, with 100% of the infected mosquitoes ultimately exhibiting a disseminated infection, regardless of the virus dose. Transmission rate data revealed a time-dependent, but overall weak, transmission barrier, with individuals transmitting as soon as 2 days post-exposure (dpe) and >50% infectious mosquitoes at 6 dpe for the highest dose. Based on these experimental intra-vector dynamics data, epidemiological simulations conducted with an agent-based model showed that even at low mosquito biting rates, CHIKV could trigger outbreaks locally. Together, this reveals the epidemic potential of CHIKV upon transmission by Aedes albopictus in mainland France.

Published online:
DOI: 10.24072/pcjournal.326
Type: Research article
Mots-clés : Arbovirus; vector; mosquito; Aedes albopictus; chikungunya virus; epidemiology; vector competence; modelisation

Viginier, Barbara 1; Cappuccio, Lucie 1; Garnier, Céline 1; Martin, Edwige 2; Maisse, Carine 1; Valiente Moro, Claire 2; Minard, Guillaume 2; Fontaine, Albin 3, 4, 5; Lequime, Sébastian 6; Ratinier, Maxime 1; Arnaud, Frédérick 1; Raquin, Vincent 1

1 IVPC UMR754, INRAE, Universite Claude Bernard Lyon 1, EPHE, PSL Research University, F-69007 Lyon, France
2 Universite Claude Bernard Lyon 1, CNRS, INRAE, VetAgro Sup, UMR Ecologie Microbienne, F-69622 Villeurbanne, France
3 Unité Parasitologie et Entomologie, Département Microbiologie et maladies infectieuses, Institut de Recherche Biomédicale des Armées (IRBA), Marseille, France
4 Aix Marseille Univ, IRD, SSA, AP-HM, UMR Vecteurs–Infections Tropicales et Méditerranéennes (VITROME), Marseille, France
5 IHU Méditerranée Infection, Marseille, France
6 Cluster of Microbial Ecology, Groningen Institute for Evolutionary Life Sciences, University of Groningen, Groningen, The Netherlands
License: CC-BY 4.0
Copyrights: The authors retain unrestricted copyrights and publishing rights 
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     author = {Viginier, Barbara and Cappuccio, Lucie and Garnier, C\'eline and Martin, Edwige and Maisse, Carine and  Valiente Moro, Claire and Minard, Guillaume and Fontaine, Albin and Lequime, S\'ebastian and Ratinier, Maxime and Arnaud, Fr\'ed\'erick and Raquin, Vincent},
     title = {Chikungunya intra-vector dynamics {in~\protect\emph{Aedes} albopictus}~from {Lyon} {(France)} upon exposure to a human viremia-like dose range reveals vector barrier{\textquoteright}s permissiveness and supports local epidemic potential},
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%A Lequime, Sébastian
%A Ratinier, Maxime
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Viginier, Barbara; Cappuccio, Lucie; Garnier, Céline; Martin, Edwige; Maisse, Carine;  Valiente Moro, Claire; Minard, Guillaume; Fontaine, Albin; Lequime, Sébastian; Ratinier, Maxime; Arnaud, Frédérick; Raquin, Vincent. Chikungunya intra-vector dynamics in Aedes albopictus from Lyon (France) upon exposure to a human viremia-like dose range reveals vector barrier’s permissiveness and supports local epidemic potential. Peer Community Journal, Volume 3 (2023), article  no. e96. doi : 10.24072/pcjournal.326. https://peercommunityjournal.org/articles/10.24072/pcjournal.326/

PCI peer reviews and recommendation, and links to data, scripts, code and supplementary information: 10.24072/pci.infections.100091

Conflict of interest of the recommender and peer reviewers:
The recommender in charge of the evaluation of the article and the reviewers declared that they have no conflict of interest (as defined in the code of conduct of PCI) with the authors or with the content of the article.

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