Section: Forest & Wood Sciences
Topic: Cell biology, Plant biology, Physiology

Mechanical characterisation of the developing cell wall layers of tension wood fibres by Atomic Force Microscopy

10.24072/pcjournal.138 - Peer Community Journal, Volume 2 (2022), article no. e39.

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Trees generate mechanical stresses at periphery of stem and branches to improve their strength and to control the orientation of their axes. This key factor in the biomechanical design of trees, named “maturation stress”, occurs in wood fibres during cellular maturation when their secondary cell wall thickens. In this study, the spatial and temporal stiffening kinetics of the different cell wall layers were recorded during fibre maturation on a sample of poplar tension wood using atomic force microscopy. The thickening of the different layers was also recorded. The stiffening of the CML, S1 and S2-layers was initially synchronous with the thickening of the S2-layer and continued a little after the S2-layer reached its final thickness as the G-layer began to develop. In contrast, the global stiffness of the G-layer, which initially increased with its thickening, was close to stable long before it reached its final maximum thickness. A limited radial gradient of stiffness was observed in the G-layer, but it decreased sharply on the lumen side, where the new sub-layers are deposited during cell wall thickening. Although very similar at the ultrastructural and biochemical levels, the stiffening kinetics of the poplar G-layer appears to be very different from that described in maturing bast fibres.

Published online:
DOI: 10.24072/pcjournal.138
Type: Research article
Arnould, Olivier 1; Capron, Marie 1; Ramonda, Michel 2; Laurans, Françoise 3; Alméras, Tancrède 1; Pilate, Gilles 3; Clair, Bruno 1

1 LMGC, University of Montpellier, CNRS, Montpellier, France
2 CTM, University of Montpellier, Montpellier, France
3 INRAE, ONF, BioForA, Orléans, France
License: CC-BY 4.0
Copyrights: The authors retain unrestricted copyrights and publishing rights
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     title = {Mechanical characterisation of the developing cell wall layers of tension wood fibres by {Atomic} {Force} {Microscopy}},
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%A Ramonda, Michel
%A Laurans, Françoise
%A Alméras, Tancrède
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%A Clair, Bruno
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Arnould, Olivier; Capron, Marie; Ramonda, Michel; Laurans, Françoise; Alméras, Tancrède; Pilate, Gilles; Clair, Bruno. Mechanical characterisation of the developing cell wall layers of tension wood fibres by Atomic Force Microscopy. Peer Community Journal, Volume 2 (2022), article  no. e39. doi : 10.24072/pcjournal.138.

Peer reviewed and recommended by PCI : 10.24072/pci.forestwoodsci.100007

Conflict of interest of the recommender and peer reviewers:
The recommender in charge of the evaluation of the article and the reviewers declared that they have no conflict of interest (as defined in the code of conduct of PCI) with the authors or with the content of the article.

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