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Section: Genomics
Topic: Genetics/Genomics, Evolution

Chromosome-level genome assembly and annotation of two lineages of the ant Cataglyphis hispanica: stepping stones towards genomic studies of hybridogenesis and thermal adaptation in desert ants

Darras, Hugo1, 2  ; De Souza Araujo, Natalia1, 3 ; Baudry, Lyam4, 5; Guiglielmoni, Nadège1, 6; Lorite, Pedro7; Marbouty, Martial5; Rodriguez, Fernando8; Arkhipova, Irina8; Koszul, Romain5; Flot, Jean-François1, 3; Aron, Serge1
1 Unit of Evolutionary Biology & Ecology, Université libre de Bruxelles (ULB), Brussels, Belgium
2 Department of Ecology and Evolution, University of Lausanne, Lausanne, Switzerland
3 Interuniversity Institute of Bioinformatics in Brussels – (IB) Brussels, Belgium
4 Centre for Integrative Genomics, University of Lausanne, Lausanne, Switzerland
5 Unité Régulation Spatiale des Génomes, CNRS, Institut Pasteur, Paris, France
6 Universität zu Köln, Köln, Germany
7 Department of Biologia Experimental, Universidad de Jaén, Jaén, Spain
8 Marine Biological Laboratory, Woods Hole, Massachusetts, USA

10.24072/pcjournal.140 - Peer Community Journal, Volume 2 (2022), article no. e40.

Get full text PDF Peer reviewed and recommended by PCI

Cataglyphis are thermophilic ants that forage during the day when temperatures are highest and sometimes close to their critical thermal limit. Several Cataglyphis species have evolved unusual reproductive systems such as facultative queen parthenogenesis or social hybridogenesis, which have not yet been investigated in detail at the molecular level. We generated high-quality genome assemblies for two hybridogenetic lineages of the Iberian ant Cataglyphis hispanica using long-read Nanopore sequencing and exploited chromosome conformation capture (3C) sequencing to assemble contigs into 26 and 27 chromosomes, respectively. Further karyotype analyses confirm this difference in chromosome numbers between lineages; however, they also suggest it may not be fixed among lineages. We obtained transcriptomic data to assist gene annotation and built custom repeat libraries for each of the two assemblies. Comparative analyses with 19 other published ant genomes were also conducted. These new genomic resources pave the way for exploring the genetic mechanisms underlying the remarkable thermal adaptation and the molecular mechanisms associated with transitions between different genetic systems characteristic of the ant genus Cataglyphis.

Published online:
DOI: 10.24072/pcjournal.140
Type: Research article
Darras, Hugo 1, 2; De Souza Araujo, Natalia 1, 3; Baudry, Lyam 4, 5; Guiglielmoni, Nadège 1, 6; Lorite, Pedro 7; Marbouty, Martial 5; Rodriguez, Fernando 8; Arkhipova, Irina 8; Koszul, Romain 5; Flot, Jean-François 1, 3; Aron, Serge 1

1 Unit of Evolutionary Biology & Ecology, Université libre de Bruxelles (ULB), Brussels, Belgium
2 Department of Ecology and Evolution, University of Lausanne, Lausanne, Switzerland
3 Interuniversity Institute of Bioinformatics in Brussels – (IB) Brussels, Belgium
4 Centre for Integrative Genomics, University of Lausanne, Lausanne, Switzerland
5 Unité Régulation Spatiale des Génomes, CNRS, Institut Pasteur, Paris, France
6 Universität zu Köln, Köln, Germany
7 Department of Biologia Experimental, Universidad de Jaén, Jaén, Spain
8 Marine Biological Laboratory, Woods Hole, Massachusetts, USA
License: CC-BY 4.0
Copyrights: The authors retain unrestricted copyrights and publishing rights 
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     author = {Darras, Hugo and De Souza Araujo, Natalia and Baudry, Lyam and Guiglielmoni, Nad\`ege and Lorite, Pedro and Marbouty, Martial and Rodriguez, Fernando and Arkhipova, Irina and Koszul, Romain and Flot, Jean-Fran\c{c}ois and Aron, Serge},
     title = {Chromosome-level genome assembly and annotation of two lineages of the ant {\protect\emph{Cataglyphis} hispanica}: stepping stones towards genomic studies of hybridogenesis and thermal adaptation in desert ants},
     journal = {Peer Community Journal},
     eid = {e40},
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Darras, Hugo; De Souza Araujo, Natalia; Baudry, Lyam; Guiglielmoni, Nadège; Lorite, Pedro; Marbouty, Martial; Rodriguez, Fernando; Arkhipova, Irina; Koszul, Romain; Flot, Jean-François; Aron, Serge. Chromosome-level genome assembly and annotation of two lineages of the ant Cataglyphis hispanica: stepping stones towards genomic studies of hybridogenesis and thermal adaptation in desert ants. Peer Community Journal, Volume 2 (2022), article  no. e40. doi : 10.24072/pcjournal.140. https://peercommunityjournal.org/articles/10.24072/pcjournal.140/

Peer reviewed and recommended by PCI : 10.24072/pci.genomics.100017

Conflict of interest of the recommender and peer reviewers:
The recommender in charge of the evaluation of the article and the reviewers declared that they have no conflict of interest (as defined in the code of conduct of PCI) with the authors or with the content of the article.

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