Evolutionary Biology

Phylogeographic breaks and how to find them: An empirical attempt at separating vicariance from isolation by distance in a lizard with restricted dispersal

10.24072/pcjournal.301 - Peer Community Journal, Volume 3 (2023), article no. e74.

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Aim: Discontinuity in the distribution of genetic diversity (often based on mtDNA) is usually interpreted as evidence for phylogeographic breaks, underlying vicariant units. However, a misleading signal of phylogeographic break can arise in the absence of barrier to gene flow, under mechanisms of isolation by distance (IBD). How and under which conditions phylogeographic breaks can be reliably differentiated from populations evolving under IBD remain unclear. Here, we use multi-locus sequence data from a widely distributed lizard species to address these questions in an empirical setting. Location: Morocco. Taxon: Spiny-footed lizard (Acanthodactylus erythrurus), Squamata: Lacertidae. Methods: Using 325 samples from 40 localities, we identified genetic discontinuities within A. erythrurus based on a mitochondrial fragment and nine nuclear markers independently. Using the nuclear markers, we then applied linear regression models to investigate whether genetic divergence could be explained by geographical distances alone, or barriers to gene flow (real phylogeographic breaks). Results: A. erythrurus is characterized by an important mitochondrial diversity, with 11 strongly supported phylogeographic lineages with a crown age of 6 Mya. Nuclear markers, however, yielded weak phylogenetic support for these lineages. Using clustering methods based on genotypes at nine nuclear loci, we identified phylogeographic clusters that were partly discordant with the mtDNA lineages. Tests of IBD delimited at least four groups of populations separated by barriers to gene flow, but unambiguous separation of vicariance from IBD remained challenging in several cases. Main conclusions: The genetic diversity of A. erythrurus originates from a mix of IBD and vicariance, which were difficult to distinguish, and resulted in similar levels of mitochondrial differentiation. These results highlight that phylogeographic breaks inferred from mitochondrial data should be further investigated using multi-locus data and explicit testing to rule out alternative processes generating discontinuities in mitochondrial diversity, including IBD. We identified four groups of populations within A. erythrurus, separated by barriers to gene flow, but even using nine independent nuclear makers the power of our approach was limited, and further investigation using genome-wide data will be required to resolve the phylogeographic history of this species.

Published online:
DOI: 10.24072/pcjournal.301
Rancilhac, Loïs 1, 2, 3; Miralles, Aurélien 4; Geniez, Philippe 5; Mendez-Aranda, Daniel 6; Beddek, Menad 1, 7; Brito, José Carlos 8, 9, 10; Leblois, Raphaël 11; Crochet, Pierre-André 1

1 CEFE, CNRS, Univ Montpellier, EPHE, IRD, Montpellier, France
2 Zoologisches Institut, Technische Universität Braunschweig, Mendelssohnstr. 4, 38106, Braunschweig, Germany
3 Animal Ecology, Department of Ecology and Genetics, Evolutionary Biology Centre, Uppsala University, Norbyvägen 18D, 75236 Uppsala, Sweden
4 Institut de Systématique, Evolution, Biodiversité (ISYEB), Muséum national d’Histoire naturelle, CNRS, Sorbonne Université, EPHE, Université des Antilles, Paris, France
5 CEFE, Univ Montpellier, CNRS, EPHE-PSL University, IRD, Biogéographie et Ecologie des Vertébrés, Montpellier, France
6 Max Delbrueck Center for Molecular Medicine in the Helmholtz Association, Robert-Roessle-Str. 10, 13092, Berlin, Germany
7 Naturalia Environnement, Site Agroparc, Avignon, France
8 CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO Laboratório Associado, Campus de Vairão, Universidade do Porto, Vairão, Portugal
9 BIOPOLIS Program in Genomics, Biodiversity and Land Planning, CIBIO, Campus de Vairão, Vairão, Portugal
10 Department of Biology, Faculty of Sciences, University of Porto, Porto, Portugal
11 CBGP, INRAE, CIRAD, IRD, Institut Agro, Montpellier SupAgro, Univ. Montpellier, Montferrier-sur-Lez Cedex, France
License: CC-BY 4.0
Copyrights: The authors retain unrestricted copyrights and publishing rights
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     title = {Phylogeographic breaks and how to find them: {An} empirical attempt at separating vicariance from isolation by distance in a lizard with restricted dispersal},
     journal = {Peer Community Journal},
     eid = {e74},
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Rancilhac, Loïs; Miralles, Aurélien; Geniez, Philippe; Mendez-Aranda, Daniel; Beddek, Menad; Brito, José Carlos; Leblois, Raphaël; Crochet, Pierre-André. Phylogeographic breaks and how to find them: An empirical attempt at separating vicariance from isolation by distance in a lizard with restricted dispersal. Peer Community Journal, Volume 3 (2023), article  no. e74. doi : 10.24072/pcjournal.301. https://peercommunityjournal.org/articles/10.24072/pcjournal.301/

Peer reviewed and recommended by PCI : 10.24072/pci.evolbiol.100634

Conflict of interest of the recommender and peer reviewers:
The recommender in charge of the evaluation of the article and the reviewers declared that they have no conflict of interest (as defined in the code of conduct of PCI) with the authors or with the content of the article.

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