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Section: Infections
Topic: Biology of interactions, Microbiology, Genetics/Genomics

Longitudinal Survey of Astrovirus infection in different bat species in Zimbabwe: Evidence of high genetic Astrovirus diversity

Chidoti, Vimbiso1 ; De Nys, Hélène2, 3, 4 ; Abdi, Malika5 ; Mashura, Getrude1 ; Pinarello, Valérie1, 2, 3, 4 ; Chiweshe, Ngoni4 ; Matope, Gift1 ; Guerrini, Laure2, 3 ; Pfukenyi, Davies1, 6 ; Cappelle, Julien2, 3 ; Mwandiringana, Ellen1 ; Missé, Dorothée2 ; Gori, Elizabeth1, 7 ; Bourgarel, Mathieu2, 3, 4 ; Liégeois, Florian1, 5
1 Faculty of Veterinary Science-Biotechnology Center, University of Zimbabwe, P.O. Box MP167 Harare, Zimbabwe
2 ASTRE, Univ Montpellier, CIRAD, INRAE, Montpellier, France
3 CIRAD, UMR ASTRE, Montpellier, France
4 CIRAD, UMR ASTRE, Harare, Zimbabwe
5 MIVEGEC, University of Montpellier, IRD, CNRS, 34394 Montpellier, France
6 Faculty of Animal and Veterinary Sciences, Botswana University of Agriculture and Natural Resources, Private Bag 0027, Gaborone, Botswana
7 Department of Biochemistry, Molecular Biology and Genetics, University of Rwanda, Rwanda

10.24072/pcjournal.340 - Peer Community Journal, Volume 3 (2023), article no. e106.

Get full text PDF Peer reviewed and recommended by PCI

Astroviruses (AstVs) have been discovered in over 80 animal species including diverse bat species and avian species. A study on Astrovirus circulation and diversity in different insectivorous and frugivorous chiropteran species roosting in trees, caves and building basements was carried out at 11 different sites across Zimbabwe. Pooled and individual faecal sampling methods were used for this study, with collection dates ranging from June 2016 to July 2021. In two sites, Magweto and Chirundu, sampling was carried out at monthly intervals from August 2020 to July 2021. Astroviruses and bat mitochondrial genes were amplified using pan-AstVs and CytB /12S RNA PCR systems respectively. Phylogenetic analysis of the RdRp Astrovirus sequences revealed a high genetic diversity of astroviruses. All the bat astroviruses tested in this study clustered with the Mamastrovirus genus. Two distinct groups of amplified sequences were identified. One group was comprised of sequences isolated from Hipposideros, Rhinolophus and Eidolon helvum spp. clustered with Human Astrovirus strains: HuAstV types1-6, HuAstV-MLB1-3 and HuAstV-VA-1. The second group comprising the majority of the sequences clustered with different strains of Bat AstVs. Results from the longitudinal study at Magweto and Chirundu showed an overall AstV prevalence of 13.7% and 10.4% respectively. Peaks of AstV infection at Chirundu coincided with the period when juveniles are 4-6 months old. By combining data from our previous work on Coronaviruses, we also anaylzed co-infection of bats with Coronaviruses and Astroviruses at Magweto and Chirundu sites for which the prevalence of co-infection was 2.6% and 3.5% respectively.

Published online:
DOI: 10.24072/pcjournal.340
Type: Research article
Keywords: Astrovirus, Bats, Zimbabwe, Phylogeny
Chidoti, Vimbiso 1; De Nys, Hélène 2, 3, 4; Abdi, Malika 5; Mashura, Getrude 1; Pinarello, Valérie 1, 2, 3, 4; Chiweshe, Ngoni 4; Matope, Gift 1; Guerrini, Laure 2, 3; Pfukenyi, Davies 1, 6; Cappelle, Julien 2, 3; Mwandiringana, Ellen 1; Missé, Dorothée 2; Gori, Elizabeth 1, 7; Bourgarel, Mathieu 2, 3, 4; Liégeois, Florian 1, 5

1 Faculty of Veterinary Science-Biotechnology Center, University of Zimbabwe, P.O. Box MP167 Harare, Zimbabwe
2 ASTRE, Univ Montpellier, CIRAD, INRAE, Montpellier, France
3 CIRAD, UMR ASTRE, Montpellier, France
4 CIRAD, UMR ASTRE, Harare, Zimbabwe
5 MIVEGEC, University of Montpellier, IRD, CNRS, 34394 Montpellier, France
6 Faculty of Animal and Veterinary Sciences, Botswana University of Agriculture and Natural Resources, Private Bag 0027, Gaborone, Botswana
7 Department of Biochemistry, Molecular Biology and Genetics, University of Rwanda, Rwanda
License: CC-BY 4.0
Copyrights: The authors retain unrestricted copyrights and publishing rights 
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     author = {Chidoti, Vimbiso and De Nys, H\'el\`ene and Abdi, Malika and Mashura, Getrude and Pinarello, Val\'erie and Chiweshe, Ngoni and Matope, Gift and Guerrini, Laure and Pfukenyi, Davies and Cappelle, Julien and Mwandiringana, Ellen and Miss\'e, Doroth\'ee and Gori, Elizabeth and Bourgarel, Mathieu and Li\'egeois, Florian},
     title = {Longitudinal {Survey} of {Astrovirus} infection in different bat species in {Zimbabwe:} {Evidence} of high genetic {Astrovirus} diversity},
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Chidoti, Vimbiso; De Nys, Hélène; Abdi, Malika; Mashura, Getrude; Pinarello, Valérie; Chiweshe, Ngoni; Matope, Gift; Guerrini, Laure; Pfukenyi, Davies; Cappelle, Julien; Mwandiringana, Ellen; Missé, Dorothée; Gori, Elizabeth; Bourgarel, Mathieu; Liégeois, Florian. Longitudinal Survey of Astrovirus infection in different bat species in Zimbabwe: Evidence of high genetic Astrovirus diversity. Peer Community Journal, Volume 3 (2023), article  no. e106. doi : 10.24072/pcjournal.340. https://peercommunityjournal.org/articles/10.24072/pcjournal.340/

PCI peer reviews and recommendation, and links to data, scripts, code and supplementary information: 10.24072/pci.infections.100085

Conflict of interest of the recommender and peer reviewers:
The recommender in charge of the evaluation of the article and the reviewers declared that they have no conflict of interest (as defined in the code of conduct of PCI) with the authors or with the content of the article.

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